关键词: 茄子, 青枯病, 信号基因, SA, VIGS

Abstract:

To identify the function of signal gene in the eggplant bacterial wilt-resistance，the transcription of 14 genes were silenced by virus induced gene silencing（VIGS）in bacterial wilt resistant eggplant‘E-31’. After the VIGS eggplant was challenged by Ralstonia solanacearum，qRT-PCR result showed that the expression levels of 14 genes was reduced compared with the control. The inoculation results showed that after the silence of MKK2，MAPK6，PAD4，NPR1，SGT1，TGA，GluA and WRKY70 in the resistant eggplant，the eggplant displayed the wilt symptom. While the expression levels reducing of MAPK3，MAPK4，NDR1，JAR1，EIN2 and EIL1 in the‘E-31’eggplant did not cause the bacterial wilt resistance down. The resulted suggested MKK2，MAPK6，PAD4，NPR1，SGT1，TGA，GluA and WRYK70 positively regulated the resistance of bacterial-wilt in the eggplant. However，MAPK3，MAPK4，NDR1， EIL1，EIN2 and JAR1 was not involved in the bacterial wilt-resistance or negatively regulated the resistance. The result indicated that the regulation of bacterial wilt-resistance involved in eggplant may mainly rely on SA signal way.

Key words: eggplant, bacterial wilt, signal gene, SA, VIGS