关键词: 苹果, 平邑甜茶, 微酸性电解水, 连作, 微生物

Abstract:

Effects of SAEW（Slightly Acid Electrolyzed Oxidizing Water，pH 6.0）on the replanted soil environment and Malus hupeheusis Rehd. seedlings biomass were investigated in pot experiments. Results showed that SAEW significantly enhanced Malus hupeheusis Rehd. seedling biomass. Compared with the control，SAEW increased the length of roots，surface area of roots，total roots volume and root activity by 47.03%，141.79%，101.31% and 37.31%，respectively. Compared with the control，SAEW decreased the numbers of fungi by 48.1%，and the ratio of bacteria to fungi increased by 120%. T-RFLP（Terminal Restriction Fragment Length Polymorphism）was applied in the analysis of soil fungal  diversity. Based on the T-RFLP，diversity index analysis，cluster analysis and principal component analysis，the simpson index of SAEW on the replanted soil was improved，Shannon index，Pielou index and Margalef index were reduced significantly. However，the SAEW treatment soil expressed a reverse trend. SAEW on the replanted soil formed independent community structure，and these communities could adapt to their own specific soil environment and become the dominant population. SAEW could reduce apple replant disease.

Key words: apple, Malus hupehensis, SAEW, replant disease, microorganism