关键词: 柿, 花粉形态, 分类学, 扫描电镜

Abstract:

Pollen grains of 8 persimmon species composed of 7 known species and 1 unknown resource were collected from National Field GenBank of China. Pollen grains were examined using scanning electron microscopy，to study pollen morphology and taxonomic status. The results showed that：（1）Pollen morphology observation revealed the grains pretreated in Diospyros have three germinal furrow and the shape of grains are nearly spherical. The grain is round in polar view. Pollen size was measured by length of polar axis and equatorial axis. The value of pollen size was between 24.7 µm and 52.8 µm in polar axis and between 23.8 µm and 47.9 µm in equatorial axis. In germinal furrow，there are many granular spiral units. Exine of pollen are striped ornamentation like grain. The width of stripe and length of germinal furrow among these species exist significant differences. In addition，surface of pollen have obvious or not obvious perforations.（2）D. lotus L.，D. glaucifolia Metc.，D. oleifera Cheng. belonged to groupⅠ；Nishimurawase，D. virginiana，male strain No. 2 formed group Ⅱ；Deyangshi performed the group Ⅲ. The classification using clustering analysis is positively correlated with their ploidy levels. （3）Pollen morphology of Deyangshi is significantly different from other materials，and the results laid the foundation of palynology for taxonomic status of Deyangshi.

Key words: Diospyros, pollen morphology, taxonomy, SEM