白梨品种4个新S基因的分离与鉴定

园艺学报 ›› 2007, Vol. 34 ›› Issue (4): 859-864.

白梨品种4个新S基因的分离与鉴定

乌云塔娜^1,2；谭晓风^1,2；曹玉芬³；张　林^1,2；邓建军^1,2

(¹中南林业科技大学经济林育种与栽培国家林业局重点实验室, 长沙410004; ² 中南林业科技大学资源与环境学院,长沙410004; ³ 中国农业科学院果树研究所, 辽宁兴城125100)

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-08-25 发布日期:2007-08-25

Isolation and Identification of Four New S-RNase Genes of White Pear( Pyrus bretschneideri)

WUYUN Ta-na^1,2；TAN Xiao-feng^1,2；CAO Yu-Fen³；ZHANG Lin^1,2；DENG J ian-jun^1,2

(¹Central-south Forestry University, Key Laboratory of Non-wood Forest Product of Forestry Ministry, Changsha 410004, China;² School of Resources and Environm ent, Central-south Forestry University, Changsha 410004, China; ³ Fruit Tree Research Institute, Chinese Academy of Agricultural Sciences, Xingcheng, Liaoning 125100, China)

Received:1900-01-01 Revised:1900-01-01 Online:2007-08-25 Published:2007-08-25

摘要/Abstract

摘要： 利用S_1-8-RNase氨基酸保守序列‘FTQQYQ’和‘IIWPNV’设计兼并引物, 对白梨4个品种
冬黄、新梨一号、红皮酥和博山池的基因组DNA进行PCR扩增。PCR产物限制性酶切分析显示: 新梨一号和冬黄各含有一个S₈ - 等位基因。DNA序列测定和生物信息学分析表明, 这4个品种中含有与已报道的S_1-19等位基因有差异的S基因, 经分析鉴定为新的S 等位基因, 分别命名为S₂₀ - 、S₂₂ - 、S₂₆ - 、S₂₇ - 等位基因(GenBank登录号分别为: AY250988、AY250990、AY339396、AY339397) 。冬黄、新梨一号、红皮酥和博山池的S基因型分别为S₈ S₂₀、S₈ S₂₂、S₁₂ S₂₆和S₁₉ S₂₇。

关键词: 关键词: 白梨, 自交不亲和性, S - 等位基因, S基因型

Abstract: PCRs were performed on genomic DNAs of four Chinese white pear (Pyrus bretschneideri) culti
varsDonghuang, Xinliyihao, Hongp isu and Boshanchi using degenerate primers derived from conserved amino
acids FTQQYQ and anti-IIWPNV of Japanese pear S_1-8-RNases. Restricted digestion analysis of PCR products
revealed that Xinliyihao and Donghuang had a common S₈
-allele. Sequencing and bioinformatics analysis of un
digested PCR p roduct showed that four alleles from the four cultivarswere distincted from published S₁_-19-alleles
at the DNA and deduced amino acid sequences level, thereby being identified as new alleles. Theywere named
as S₂₀-, S₂₂-, S₂₆- and S₂₇-alleles, respectively ( GenBank accession numbers AY250988, AY250990,
AY339396 and AY339397). Finally, the four cultivarswere S2genotyped as follows: Donghuang (S₈ S₂₀ ) , Xin
liyihao (S₈ S₂₂) , Hongp isu (S₁₂ S₂₆ ) and Boshanchi (S₁₉ S₂₇ ).

Key words: Pyrus bretschneideri, Self-incompatibility, S-allele, S-genotype

乌云塔娜;谭晓风;曹玉芬;张　林;邓建军;. 白梨品种4个新S基因的分离与鉴定[J]. 园艺学报, 2007, 34(4): 859-864.

WUYUN Ta-na;TAN Xiao-feng;CAO Yu-Fen;ZHANG Lin;DENG J ian-jun;. Isolation and Identification of Four New S-RNase Genes of White Pear( Pyrus bretschneideri)[J]. ACTA HORTICULTURAE SINICA, 2007, 34(4): 859-864.

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