关键词: 花烛, 体细胞胚, 诱导, 发育, 植株再生

Abstract: A protocol for somatic embryogenesis and plant regeneration of Anthurium andraeanum Lind. in vitro plants has been successfully developed. Leaf sections, petioles and root sections were used as explants, the results showed that leaf sectionswere suitable exp lants and expressed higher embryogenic potential than others; Up to 9013% of explants produced embryogenic calli and embryogenic structures after 40 days culture on modified MS medium supplemented with 2.0 to 3.0 mg·L ^-1 2, 4-D, 0.5 mg·L ^-1 KT, 2% sucrose and 1% glucose; 62.9% of somatic embryos developed after 20 days culture on modified MS medium containing 0.5 mg·L ^{- 1} 6-BA and 3% sucrose under light conditions of 20 μmol·m^{- 2} ·s^-1 and converted into plantlets with shoots and roots after 30 days culture on the same medium. The observation of histological structure and ultrastructure proved the process of somatic embryogenesis of Anthurium.

Key words: Anthurium andraeanum, Somatic embryo, Induction, Development, Plant regeneration