梨新品种及其亲本的AFLP分析

园艺学报 ›› 2007, Vol. 34 ›› Issue (4): 847-852.

梨新品种及其亲本的AFLP分析

王　斐;林盛华;方成泉^*;李连文

(中国农业科学院果树研究所, 辽宁兴城125100)

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-08-25 发布日期:2007-08-25

AFLP Analysis of New Pear Cultivars and Their Parents

WANG Fei;LIN Sheng-hua;FANG Cheng-quan^*;LI Lian-wen

(Research Institute of Pomology, Chinese Academy of Agricultural Sciences, X ingcheng, Liaoning 125100, China)

Received:1900-01-01 Revised:1900-01-01 Online:2007-08-25 Published:2007-08-25

摘要/Abstract

摘要： 利用荧光AFLP技术, 对20个梨新品种及其23个亲本(共43个品种) 进行研究。从64对
引物组合中筛选出7对用于扩增, 共获得784条带, 其中多态性带699条, 多态性为89.2%。扩增结果显
示, 7对引物组合在29个品种中扩增出特征带, 每对引物组合均能将所有品种鉴别开, 表明荧光AFLP技
术用于梨品种鉴定的效率很高。通过聚类, 从分子水平对梨新品种及其亲本的遗传关系进行分析, 并对20
个梨新品种进行分类, 为梨杂交育种的亲本选配提供了理论参考。

关键词: 梨, 品种, 鉴定, 荧光AFLP, 指纹图谱

Abstract: Forty-three pear cultivars including 20 new pear cultivars and their parentswere subjected to
fluorescent AFLP ( amplified fragments length polymorphism) analysis. Seven primer combinations selected
from 64 primer combinations produced a total of 784 fragments, of which 699 were polymorphic with a poly
morphism percentage of 89.2%. The results showed that characteristic bands were amp lified from 29 culti
vars, and any primer combination could identify all the cultivars. Fluorescent AFLP was proved to be a very
reliable method to identify pear cultivars. The genetic relationship between new pear cultivars and their parents
was analyzed and 20 new cultivars were classified on molecular level by clustered analysis, which p rovided
theoretical reference for parent selection in pear hybridization breeding.

Key words: Pyrus, Cultivar, Identification, Fluorescent AFLP, Fingerprinting

王　斐;林盛华;方成泉;李连文. 梨新品种及其亲本的AFLP分析[J]. 园艺学报, 2007, 34(4): 847-852.

WANG Fei;LIN Sheng-hua;FANG Cheng-quan;LI Lian-wen. AFLP Analysis of New Pear Cultivars and Their Parents[J]. ACTA HORTICULTURAE SINICA, 2007, 34(4): 847-852.

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