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园艺学报 ›› 2007, Vol. 34 ›› Issue (4): 853-858.

• 果树 • 上一篇    下一篇

12个梨品种S基因型的鉴定

衡 伟1;张绍铃1,*;张妤艳1;吴 俊1;李秀根2
  

  1. (1 南京农业大学园艺学院, 南京210095; 2 中国农业科学院郑州果树研究所, 郑州450009)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-08-25 发布日期:2007-08-25

Identification of S-genotypes of Twelve Pear Cultivars by Analysis of DNASequence

HENG Wei1;ZHANG Shao-ling1,*;ZHANG Yu-yan1;WU Jun1;LI Xiu-gen2   

  1. (1 College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China; 2 Zhengzhou Fruit Research Institute,Chinese Academy Agricultural Sciences, Zhengzhou 450009,China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-08-25 Published:2007-08-25

摘要: 应用PCR扩增技术, 根据目的S基因与GenBank中已知S 基因同源性100%原理鉴定了我国
育成的12个梨品种的S基因型, 分别是: ‘红酥脆’为S4S36 , ‘新梨7号’为S28Sd , ‘金水3号’为S5S29 , ‘八月酥’为S3S16 , ‘金水1号’为S3S29 , ‘龙泉酥’为S3S22 , ‘雪花’为S4S16 , ‘雪芳’为S4S16 ,
‘雅青’为S4S34 , ‘新雅’为S4S34 , ‘德胜香’为S3S29 , ‘富源黄’为S16S33。通过对S基因的DNA序列分
析, 确认S16、S31为同一S基因。本研究结果丰富了我国梨品种的S基因型信息, 为田间合理配置授粉品种
提供了依据。


关键词: 梨, 自交不亲和性, DNA序列分析, S基因型

Abstract: Using PCR amplification method, S-genotypes of twelve Chinese pear cultivars were deter
mined. The S-genotypeswere S4S36 for Hongsucui, S28Sd for Xinli 7, S5S29 for Jinshui 3, S3S16 for Bayuesu,
S3S29 for Jinshui 1, S3S22 for Longquansu, S4S16 for Xuehua, S4S16 for Xuefang, S4S34 for Yaqing, S4S34 for
Xinya, S3S29 forDeshengxiang, S31S33 for Fuyuanhuang. Based on the analysis of the S gene, Itwas confirmed
that S16 and S31 were the same S gene. The study results enriched the genotype information of pear cultivars in
China, p roviding the basis for app rop riate arrangement of pollination tree in pear orchards.

Key words: Pear, Pyrus, Self-incompatibility, Analysis of DNA sequence, S-genotype