关键词: 月季, 抑制性消减杂交, 花色, 花香, 突变体, 差异表达

Abstract: In order to analyse molecular mechanism of rose flower and scent metabolism and to exp lore
the genes involved in rose floral color and fragrance metabolism, the supp ressive subtraction hybridization
( SSH) technique was used. Two differential exp ressing cDNA libraries were constructed with the cDNAs of
the rose color and scentmutantWangriqinghuai as driver and the cDNAs of itswild type J inyindao (Rosa ×hy
brida) as tester, vice versa. The cDNA libraries ofWSSH indicated the differential exp ressing cDNA frag
ments in Wangriqinghuai and JSSH indicated the differential exp ressing cDNA fragments in Jinyindao. The
difference of PCR bands for substracted samp le and non-substracted samp le was obvious. The relevant cDNA
fragments exp ressing differentially between Wangriqinghuai and J inyindao were revealed and isolated through
micoarray analysis of the clones in SSH. Twenty-seven positive clones ofWSSH and 25 positive clones of JSSH
were obtained. After sequencing the clones exp ressing differentially in two cultivars and blasting their se
quences, some ESTs regulating the color of pink mutant, such as CHS, DFR, MYB1, ADR were obtained
from WSSH. Some ESTs regulating the floral color and scent of yellow p rogenitor, such as OOMT, MYB92 were also obtained from JSSH. Ty12cop ia2like retrotransposon most likely causing bud mutation was also got in
this study. This is the first report on rose mutant SSH which would be useful for analysis and isolation of the
genes related to rose floral color or scent.

Key words: Supp ressive subtraction hybridization, Floral color, Floral scent, Mutant, Differentialexpression