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园艺学报 ›› 2016, Vol. 43 ›› Issue (3): 515-524.doi: 10.16420/j.issn.0513-353x.2015-0434

• 观赏植物 • 上一篇    下一篇

冬樱花扦插苗与实生苗对不同形态无机氮素吸收的动力学特征分析

朱开元,刘慧春*,周江华,邹清成,马广莹,张加强   

  1. 浙江省农业科学院花卉研究开发中心,杭州 311202
  • 出版日期:2016-03-25 发布日期:2016-03-25
  • 基金资助:
    杭州市种子种苗专项(20130932H20);浙江省公益性研究农业项目(2013C32088)

Kinetic Characteristics of Inorganic Nitrogen Uptake by Cerasus cerasoides Cuttings and Seedlings

ZHU Kai-yuan,LIU Hui-chun*,ZHOU Jiang-hua,ZOU Qing-cheng,MA Guang-ying,and ZHANG Jia-qiang   

  1. Flower Research and Development Center,Zhejiang Academy of Agricultural Sciences,Hangzhou 311202,China
  • Online:2016-03-25 Published:2016-03-25

摘要: 以冬樱花(Cerasus cerasoides[D. Don]Sok)扦插苗和实生苗为试材,研究其在水培条件下对外源铵态N(氮)和硝态N比例(5︰0、4︰1、3︰2、2︰3、1︰4)处理中铵态N、硝态N和总无机N素在24 h后的吸收动力学响应。结果表明:随着外源铵:硝比例的降低,铵态N的净流速逐渐下降,硝态N的净流速逐渐上升,扦插苗吸收的总无机N素净流速在外源铵︰硝比为3︰2时达到最大(6.70 ± 0.34)μmol ? h-1后下降,而实生苗在外源铵︰硝比为1︰4时达到最大(14.88 ± 1.99)μmol ? h-1;总体上,总无机N素进入扦插苗的平均净流速[(4.76 ± 1.29)μmol ? h-1]低于实生苗[(12.95 ± 1.35)μmol ? h-1](P < 0.05),但两种苗木间净吸收速率差异不显著(32 ~ 34 μmol ? h-1 ? g-1)。养分吸收动力学参数拟合结果显示,扦插苗更加偏好于吸收硝态N,实生苗则更加偏好于吸收铵态N,但当外源铵硝比小于4︰1时,两种苗木同时偏向于对硝态N的吸收。控制外源铵硝比例在3︰2可以促进冬樱花扦插苗对无机N素的吸收,而控制外源铵硝比例在2︰3以下则更有利于实生苗对无机N素的吸收。

关键词: 冬樱花, 铵态氮, 硝态氮, 扦插苗, 根系

Abstract: Cuttings and seedlings of Cerasus cerasoides[D. Don]Sok were soaked for 24 h under hydroponic condition,where exogenous ammonium-nitrogen(N)and nitrate-N were supplied at concentration concentration-ratios of 5︰0,4︰1,3︰2,2︰3,and 1︰4. Thereafter,materials both seedlings and cuttings were sampled to detect their responses of N uptake kinetics. Results revealed that:with the decline of concentration concentration-ratio of ammonium to nitrate,net flux rate of ammonium-N decreased but that of nitrate-N increased,whilst net flux rate of total inorganic N for cuttings increased to be [(6.70 ± 0.34)μmol ? h-1] until the concentration ratio of 3︰2,and that for seedlings increased to be [(14.88 ± 1.99)μmol ? h-1] at the concentration ratio of 1︰4;Overall,net flux rate of total inorganic N incuttings[(4.76 ± 1.29)μmol ? h-1]was lower than that in seedlings[(12.95 ± 1.35)μmol ? h-1](P < 0.05),but the difference of net uptake rate of N ions between cuttings and seedlings(32–34 μmol ? h-1 ? g-1)was not significant;Estimated parameters of nutrient uptake kinetics indicated that cuttings preferred the uptake of nitrate-N,but seedlings preferred the uptake of ammonium-N. However,when the concentration-ratio of ammonium to nitrate was lower than 4︰1 both propagation types preferred the uptake of nitrate-N. In conclusion,manipulations controlling the concentration ratio of ammonium to nitrate around 3︰2 could promote the uptake of inorganic N by cuttings,and the ratio of 2︰3 would benefit for the uptake of N by seedlings.

Key words: Cerasus cerasoides, ammonium, nitrate, cutting, root

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