关键词: 苹果, MdEIL1, 表达分析, 原核表达, 功能鉴定

Abstract:

An ethylene signal relative transcription factor named MdEIL1 was cloned from Malus × domestic‘Royal Gala’. Sequence analysis showed that MdEIL1 contained 1 980 nucleotides，which encoded a protein containing 658 amino acids. Phylogenetic tree analysis showed that MdEIL1 belonged to EIN3/EIL transcription factor family proteins. qRT-PCRs analysis indicated that MdEIL1 has different levels of expression in apple’s roots，stems，leaves，flowers and fruits，and was induced by ethylene，auxin and cytokinin hormones hormones in plants. We obtained the recombinant MdEIL1 protein by Prokaryotic expression，which was essential for protein functional identification in vitro. Ectopic expression of MdEIL1 in Arabidopsis can partially enhance ethylene response，which exhibited the short hypocotyl in darkness and expression of AtERF1 was improved in the MdEIL1 overexpressing transgenic lines.

Key words: apple, MdEIL1, expression analysis, prokaryotic expression, functional identification