关键词: 茉莉花, 萜类合成酶, 实时荧光定量PCR, 原核表达

Abstract: The full length cDNA of terpene synthase gene（JsTPS）was cloned by combination of RT-PCR and RACE from petals of Jasminum sambac. The results showed that full length cDNA contained 1 491 bp including an 1 884 bp ORF，encoding a 56 989.7 D protein with 496 amino acids whose molecular weight was consistent to that of product of the gene by prokaryotic expression；The result of alignment of amino acid showed that the gene had a homology of 75% to that of olive（Olea europaea），belonging to α-Farnesene synthases；Quantities of the gene were detected by real-time PCR in process of opening of flower，the results showed that the expressing level of the gene was minimum at 18：00 when the flowers were not open，then had been increasing until at 22：00 when the expressing level reached to maximum and the flowers was opening.

Key words: Jasminum sambac, terpene synthase gene, quantitative real-time PCR, prokaryotic expression