石榴采后腐烂病病原菌的分子鉴定

园艺学报 ›› 2007, Vol. 34 ›› Issue (4): 877-882.

石榴采后腐烂病病原菌的分子鉴定

付娟妮;刘兴华^*;蔡福带;寇莉萍

(西北农林科技大学食品科学与工程学院, 陕西杨凌712100)

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-08-25 发布日期:2007-08-25
通讯作者: 刘兴华

Identification of Pathogenic Fungus Causing a Decay of Stored Pomegranate Fruits Using Molecular Biology Technique

FU Juan-ni;LIU Xing-hua^*;CAI Fu-dai;KOU Li-ping

( Food Science and Engineering College, Northwest A & F University, Yangling, Shaanxi 712100, China)

Received:1900-01-01 Revised:1900-01-01 Online:2007-08-25 Published:2007-08-25
Contact: LIU Xing-hua

摘要/Abstract

摘要： 按照柯赫氏法则对石榴采后腐烂病病原菌进行了分离, 应用PCR技术对分离菌rDNA的内转
录间隔区( ITS区) 基因克隆后测序, 并将所测序列提交到欧洲分子生物学实验室核酸数据库( European
Molecular Biology Laboratory Genbank) , 通过Blast程序将该序列与数据库已有的序列进行分析比较, 发现与
葡萄座腔菌(Botryosphaeria dothidea) 的同源性达到了99%以上, 因此认为石榴腐烂的病原菌是葡萄座腔
菌。该菌引起的石榴腐烂病害特征与以往报道的石榴干腐病相似。对葡萄座腔菌的部分生物学特性和侵染
循环规律进行了描述。

关键词: 石榴, 葡萄座腔菌, 贮藏, ITS - rDNA测序

Abstract: According to Koch'postulates, the pathogenic fungus causing a destructive decay of stored
pomegranate fruitswas isolated and confirmed. After cloning the internal transcribed spacer ( ITS) region of
the isolate using polymerase chain reaction ( PCR) technique, the gene sequence of ITS region of isolate were
determined. Using Blast System, the difference of the gene sequences between the isolate and the fungus in
EuropeanMolecular B iology Laboratory Genbank was compared. The result showed that the isolate fungus and
B otryosphaeria dothidea share more 99% homology sequence. Therefore, the isolated funguswas identified as
B otryosphaeria dothidea. The symp toms of B otryosphaeria dothidea causing fruit rot are same with other fungus
in other reports. This paper is the first report about B otryosphaeria dothidea causing pomegranate rot. In addi
tion, the disease cycle and the ep idemiology of B otryosphaeria dothidea were investigated in order to control
the decay of stored pomegranate fruits.

Key words: Pomegranate, Botryosphaeria dothidea, Storage, ITS - rDNA sequencing

付娟妮;刘兴华;蔡福带;寇莉萍. 石榴采后腐烂病病原菌的分子鉴定[J]. 园艺学报, 2007, 34(4): 877-882.

FU Juan-ni;LIU Xing-hua;CAI Fu-dai;KOU Li-ping. Identification of Pathogenic Fungus Causing a Decay of Stored Pomegranate Fruits Using Molecular Biology Technique[J]. ACTA HORTICULTURAE SINICA, 2007, 34(4): 877-882.

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