关键词: 大白菜, 结球甘蓝, 易位系, InDel 标记, 游离小孢子培养

Abstract:

In order to create Chinese cabbage–Cabbage translocation lines，M2 plants obtained
through pollen radiation of Chinese cabbage–Cabbage monosomatic alien addition line AC2，were carried
out to isolated microspore culture. Six translocation lines with cabbage chromosome 2 fragment were
identified using 26 specific InDel markers corresponding cabbage chromosome 2 and cytological
observation. The exogenetic fragment for those translocation lines was further identified to contain
cabbage specific InDel markers C09-4 and C09-4-52 and the fragment size was about 788.3 kb by
additional InDel markers. Two translocation lines‘AT2-1’and‘AT2-2’from two M2 plants were selfed，
backcrossed with‘85-1’，and hybridized with Chinese cabbage inbred lines‘14-28’and‘14-36’，and the progenies were detected using the cabbage specific InDel markers C09-4 and C09-4-52 of cabbage.
The results showed that 85 selfing progenies，82 backcrossing progenies and 188 hybrids contained the two
cabbage specific markers，which indicated that the cabbage chromosome fragment could be transmitted to
the next generation stability.

Key words: Chinese cabbage, cabbage, translocation lines, InDel markers, isolated microspore
culture