茄子青枯病抗性基因的遗传分析及其AFLP标记

园艺学报 ›› 2006, Vol. 33 ›› Issue (4): 869-872.

茄子青枯病抗性基因的遗传分析及其AFLP标记

李　猛¹;王永清²;田时炳²;罗章勇²;王小佳^1*

(¹ 西南大学园艺园林学院, 重庆400716; ²重庆市农业科学研究所, 重庆400055)

收稿日期:2006-01-26 修回日期:2006-06-20 出版日期:2006-08-25 发布日期:2006-08-25

Genetic Analysis of Resistance to Bacterial Wilt and Identification of an Associated AFLP Marker in Eggplant ( Solanum melongena )

Li Meng¹;Wang Yongqing²;Tian Shibing²;Luo Zhangyong²;Wang Xiaojia^1*

(¹College of Horticulture and Landscape, Southwest University, Chongqing 400716, China; ²Chongqing Institute of Agricultural Sciences, Chongqing 400055, China)

Received:2006-01-26 Revised:2006-06-20 Online:2006-08-25 Published:2006-08-25

摘要/Abstract

摘要： 以茄子高感青枯病品种‘三月茄’与高抗品种‘S69’的F1、F2为材料, 对青枯病抗性遗传进行了分析, 同时开展了与抗性基因连锁的AFLP标记鉴定。结果表明: ‘S69’对青枯病的抗病性属于不完全隐性遗传, 感病性属于不完全显性; 抗性由1～2对基因控制。对86个F2单株AFLP分析, 鉴定出1个与‘S69’抗性基因连锁的AFLP标记39A980 (该引物组合为E-ACC /M-CTG) , 该标记与抗性基因间的交换值为4.65% , 遗传距离为4.9 cM。

关键词: 茄子, 青枯病, 抗性遗传, AFLP标记

Abstract: F₁and F₂ populations, derived from the cross between bacterialwilt highly susceptible cultivar ‘Sanyueqie’and highly resistant Indonesian cultivar‘S69’, were used for investigation of the inheritance of resistance to bacteria wilt and for identification of associated AFLP markers in eggplant. The results showed that the resistance to bacterialwiltwas incompletely recessive, otherwise susceptibilitywas incomp letely dominant, which was controlled by 1 - 2 genes. By using a combination of the amp lified fragment length polymor2
phism (AFLP) technique and bulked segregant analysis (BSA) , an AFLP marker, named 39A980 was identified with the p rimer combination E-ACC /M-CTG to be linked to the major resistant gene. The crossing-over value and the genetic distance between the resistant gene and‘39A980 ’were estimated as 4.65% and 4.9 cM respectively by analyzing a set of 86 F₂ plants.

Key words: Solanum melongena, Bacterial wilt, Inheritance of resistance, AFLP markers

李　猛;王永清;田时炳;罗章勇;王小佳. 茄子青枯病抗性基因的遗传分析及其AFLP标记[J]. 园艺学报, 2006, 33(4): 869-872.

Li Meng;Wang Yongqing;Tian Shibing;Luo Zhangyong;Wang Xiaojia. Genetic Analysis of Resistance to Bacterial Wilt and Identification of an Associated AFLP Marker in Eggplant ( Solanum melongena )[J]. ACTA HORTICULTURAE SINICA, 2006, 33(4): 869-872.

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