‘津田’芜菁消减cDNA文库的构建及分析

园艺学报 ›› 2006, Vol. 33 ›› Issue (4): 866-868.

‘津田’芜菁消减cDNA文库的构建及分析

许志茹;李玉花

(东北林业大学生命科学学院, 黑龙江哈尔滨150040)

收稿日期:2005-09-16 修回日期:2005-12-22 出版日期:2006-08-25 发布日期:2006-08-25

Construction and Analysis of‘Tsuda’Turnip Subtracted cDNA L ibrary

Xu Zhiru;Li Yuhua

(Life Science College, Northeast Forestry University, Harbin, Heilongjiang 150040, China)

Received:2005-09-16 Revised:2005-12-22 Online:2006-08-25 Published:2006-08-25

摘要/Abstract

摘要： 以‘津田’芜菁红色块根和白色块根为材料, 利用SSH技术成功构建了消减cDNA文库并富集相关基因片段。从消减文库中筛选到960个阳性克隆, PCR鉴定插入片段长度主要分布于300～800 bp之间。克隆测序后与GenBank中的同源序列进行比较, 特异基因片段为302个, 其中99个与数据库中的序列具有相似区域且功能已知, 与数据库中序列没有显著同源性及功能未知的序列为203条。

关键词: 芜菁, SSH, 消减cDNA文库, 差异表达基因

Abstract: A substracted cDNA library was constructed by using SSH technique to enrich related genes.cDNA p repared from red root peel of‘Tsuda’turnip was used as tester, and that from white oneswas used as driver. 960 positive clones were got from the library. The inserted size was between 300 and 800 base pair as it was determined by PCR method. After sequencing and clustering, 302 unique geneswere obtained. By running blastn p rogram of NCB I, 99 ESTs were found to have the homologous genes of the database with known
function, and 203 ESTs without remarkable homology or with unknown.

Key words: Turnip, SSH, Subtracted cDNA library, Differentially expressed gene

许志茹;李玉花. ‘津田’芜菁消减cDNA文库的构建及分析[J]. 园艺学报, 2006, 33(4): 866-868.

Xu Zhiru;Li Yuhua. Construction and Analysis of‘Tsuda’Turnip Subtracted cDNA L ibrary[J]. ACTA HORTICULTURAE SINICA, 2006, 33(4): 866-868.

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