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园艺学报 ›› 2007, Vol. 34 ›› Issue (4): 871-876.

• 果树 • 上一篇    下一篇

樱桃主栽品种的遗传多样性分析

艾呈祥1;辛 力1; 余贤美2;张力思1; 魏海蓉1; 苑克俊1; 孙清荣1; 刘庆忠1,3

  

  1. (1 山东省果树研究所, 山东省果树生物技术育种重点实验室, 山东泰安271000; 2 中国热带农业科学院环境与植物保护研究所, 海南儋州571737)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-08-25 发布日期:2007-08-25

Analysis of Genetic Diversity in Cerasus by SSR Markers

AI Cheng-xiang1;XIN Li1;YU Xian-mei2;ZHANG Li-si1;WE I Hai-rong1;YUAN Ke-jun1;SUN Qing-rong1;LIU Qing-zhong1,3
  

  1. (1 Shandong Institute of Pomology, Key Laboratory for Fruit Biotechnology Breeding of Shandong, Tai'an, Shandong 271000,China; 2 Institute of Environm ent and Plant Protection, Chinese Academy of Tropical Agriculture Sciences, Danzhou, Hainan571737, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-08-25 Published:2007-08-25

摘要: 利用24对SSR引物对30个樱桃主栽品种进行遗传多样性分析, 以了解樱桃产区的资源多样
性状况, 为种质资源的开发和利用提供帮助。结果表明: 樱桃产区具有丰富的遗传变异。SSR的遗传多样
性指数的分布范围为1.3647~2.9964, Simp son指数分布范围为0.6111~0.9326。30个品种的分子数据聚
类结果均呈现一定的地理分布规律。根据系统聚类分析将30个樱桃品种分为两大类: 欧洲甜樱桃和中国樱
桃, 与传统分类学的结果相符。同时在两大类内又将品种进行细分, 第Ⅰ类分为3组, 第Ⅱ类分为2组,
其结果与地理分布有一定的相关性, 能够反映樱桃的遗传特点及区域特性。

关键词: 樱桃, 品种, 遗传多样性, SSR

Abstract: The genetic diversity of thirty cultivars of Prunus avium L. and P. pseudocerasus Lindl. were
evaluated by 24 pairs of SSR p rimers. The aim of the study is to reveal the genetic diversity in Cerasus and
help to exp loit and make use of the germp lasm of Cerasus. The results showed that the Shannon2weaver index
of genetic diversity ranged from 1.3647 to 2.9964 and the Simp son index from 0.6111 to 0.9326, which indi
cated that the Cerasus has abundant genetic diversity. The molecular clustering showed some rules in the geo
graphical distribution. The cultivars used in this studywere divided into 2 group s by cluster analysis: P. avi
um L and P. pseudocerasus Lindl. , which was in agreementwith the traditional taxonomy on Cerasus. Moreo
ver, P. avium L. was subdivided into 3 subgroup s, and P. pseudocerasus L indl. into 2 subgroup s respective
ly. The cluster results showed that the molecular classification of Cerasus in China had some relativity to the
geographic taxonomic system, which reflected the genetic and territorial character of Cerasus.

Key words: Cerasus, Cultivar, Genetic diversity, SSR