关键词: 水仙, 温度诱导运载蛋白, 克隆, 表达, 夏休眠

Abstract:

An encoding temperature-induced lipocalin（TIL）gene NtTIL was separated and cloning by PCR in Narcissus tazetta var. chinensis. The open reading frame of NtTIL was 567 bp，encoding a protein of 188 amino acids. Biological software for NtTIL protein analysis showed that：The gene belongs to lipocalin-2 superfamily gene family；typical domains deduced from amino acid sequence contained SCR1，SCR2 and SCR3 three of botanical lipocalin. It’s homology greater than wheat，Arabidopsis and other plants 75%. Sequence analyses revealed that this protein was stable and hydrophilic without signal peptides；transmembrane movement was from outside to inside in the outward N-terminus，then transmembrane. And the relative expression of this gene was surveyed by real-time quantitative PCR during different growth stage. The results showed that its relative expression during different growth stage was different；the NtTIL expression was low in bulb swelling stage，expression increased and then reduced in dormancy stage，and expression increased slightly in flower differentiation stage. In conclusion，itsuggested that NtTIL could play a role for regulation of bud dormancy in Narcissus tazetta var. chinensis.

Key words: Narcissus tazetta var. chinensis, temperature-induced lipocalin, cloning, expression, dormancy