关键词: 花椰菜, miRNA, Bra-miR07, miRNA功能, 器官发育

Abstract:

In the previous study，a series of novel miRNAs that show significantly different expression in the early organ development of cauliflower were obtained by high-throughput sequencing. In the present study，a novel miRNA that showed different expression between cauliflower hypocotyl and cotyledon was named Bra-miR07 and further analyzed. The results indicated that the sequence length of Bra-miR07 precursor was 270 bp which can form the stable secondary hairpin structure. Bra-miR07 appeared higher expression in cauliflower cotyledon than that in hypocotyl. To further uncover the function of Bra-miR07，overexpression vector of Bra-miR07 was constructed and transferred into Arabidopsis thaliana by floral dip，and the Bra-miR07 overexpression transgenic plants were successfully obtained. Phenotypic analyses showed that overexpression of Bra-miR07 can significantly influence thegrowth and development of transgenic plants. Compared with wild-type Arabidopsis，Bra-miR07 transgenic plants showed slow growth，dwarf plant and lower fertility. Consequently，these results implied that Bra-miR07 may also play important roles in regulation of cauliflower organ development.

Key words: cauliflower, miRNA, Bra-miR07, miRNA function, organ development