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园艺学报 ›› 2016, Vol. 43 ›› Issue (1): 89-99.doi: 10.16420/j.issn.0513-353x.2015-0584

• 蔬菜 • 上一篇    下一篇

江苏省番茄黄化曲叶病毒和褪绿病毒复合侵染的分子检测

吴淑华1,李廷芳1,赵文浩1,程兆榜1,郭青云4,赵统敏2,余文贵2,朱叶芹3,*,季英华1,*   

  1. 1江苏省农业科学院植物保护研究所,江苏省食品质量安全重点实验室—省部共建国家重点实验室培养基地,南京 210014;2江苏省农业科学院蔬菜研究所,南京 210014;3江苏省植物保护站,南京 210036;4青海省农林科学院植物保护研究所,西宁 810016
  • 出版日期:2016-01-25 发布日期:2016-01-25
  • 基金资助:

    国家公益性行业(农业)专项(201303028);江苏省农业科技自主创新重点项目[CX(12)1004];江苏省 333 高层次人才
    培养工程科研项目(BRA2013262);国家自然科学基金项目(31572074)

Molecular Identification on Mixed Infection of Tomato yellow leaf curl virus and Tomato chlorosis virus on Tomato in Jiangsu Province

WU Shu-hua1,LI Ting-fang1,ZHAO Wen-hao1,CHENG Zhao-bang1,GUO Qing-yun4,ZHAO Tong-min2,YU Wen-gui2,ZHU Ye-qin3,*,and JI Ying-hua1,*   

  1. 1Institute of Plant Protection,Jiangsu Academy of Agricultural Sciences,Key Lab of Food Quality and Safety of Jiangsu Province – State Key Laboratory Breeding Base,Nanjing 210014,China;2Institute of Vegetable Crops,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China;3Jiangsu Station of Plant Protection,Nanjing 210036,China;4Institute of Plant Protection,Qinghai Academy of Agriculture and Forestry Sciences,Xining 810016,China
  • Online:2016-01-25 Published:2016-01-25

摘要:

2014年对江苏地区的番茄黄化曲叶病进行调查时发现,采自南京温室大棚的17份表现矮化,上部叶片上卷、变小、叶缘黄化,下部叶片脉间褪绿、上卷、变厚症状的番茄病株样本中除了感染烟粉虱传双生病毒外,还有一种长线形病毒,序列分析结果显示烟粉虱传双生病毒为番茄黄化曲叶病毒(Tomato yellow leaf curl virus,TYLCV),长线形病毒为番茄褪绿病毒(Tomato chlorosis virus,ToCV)。同时还对发病棚室中采集的烟粉虱进行了两种病毒的检测,结果两种病毒在烟粉虱体内都有检测到。

关键词: 番茄黄化曲叶病毒, 番茄褪绿病毒, 复合侵染, 分子检测

Abstract:

In 2014,a routine survey of tomato yellow leaf curl disease in Jiangsu Province was carried out. Besides whitefly-transmitted geminiviruses,a whitefly-transmitted crinivirus was also detected in 17 tomato samples collected from Nanjing with symptoms of reduced leaf size or leaf curling and yellowing in the upper leaves and interveinal chlorosis and upward rolling and thickening in the lower leaves. Sequence analysis showed that the whitefly-transmitted geminivirus was an isolate of Tomato yellow leaf curl virus(TYLCV)and the whitefly-transmitted crinivirus was an isolate of Tomato chlorosis virus(ToCV). The two viruses were also detected in Bemisia tabaci collected from the greenhouses.

Key words: Tomato yellow leaf curl virus, Tomato chlorosis virus, mixed infection, molecular detection

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