关键词: 番茄, 番茄黄化曲叶病毒, 荧光定量PCR

Abstract:

Tomato yellow leaf curl disease（TYLCD）has already been a major constraint to tomato production in China. It is caused by Tomato yellow leaf curl virus（TYLCV）and several other begomoviruses. These begomoviruses replicate their genome quickly in tomato plants after infection and transport to whole plant to result in disease. Development of TYLCV resistance variety is economic and efficient way to control TYLCD. To provide theoretical bases to the breeding for TYLCV resistance，we detected the combined effects of different TYLCV resistance genes and temperatures on TYLCV replicate TYLCV in tomato lines with Ty-1/ Ty-3 was one thousandth of that in susceptible tomato lines. However，after infiltration of infectious clone TYLCV-IL [CN：SH2] using real-time PCR. TYLCV resistance genesTy-1/ Ty-3 acted inhibitory effect on TYLCV replication in solar greenhouse in spring. The amount of TYLCV in tomato lines with Ty-1/ Ty-3 was one thousandth of that in susceptible tomato lines. However the inhibition of Ty-1/ Ty-3 on TYLCV replication was decreased in solar greenhouse in autumn. The copy number of TYLCV in tomato materials with Ty-1/ Ty-3 and susceptible tomato materials showed a similar trend of variation. Similarly，under temperature treatments，Ty-1/ Ty-3 inhibited sharply the replication of TYLCV at low temperature，but not at high temperature. In contrast to Ty-1/ Ty-3，the inhibition of Ty-2 on the replication of TYLCV was more severe and stable. The amounts of TYLCV in tomato lines with Ty-2 were one ten-thousandth of that in susceptible tomato lines in solar greenhouse both in spring and in autumn. Furthermore，there were not addictive effect between the two genes（Ty-1 and Ty-2）or among the three genes（Ty-1，Ty-2 and Ty-3）.

Key words: tomato, TYLCV, real-time PCR