关键词: 柿, DlSx-AF4S, SCAR, 雄性, 性别决定

Abstract:

Most of commercial persimmon（Diospyros kaki Thunb.）cultivars bear only female flowers and are strongly parthenocarpic. It is necessary to develop a rapid technique for identifying and elimination of male progenies which has been regarded as an important strategy for enhancing breeding efficiency. Recently，a SCAR（Sequence Characterized Amplified Region）marker DlSx-AF4S，tightly related to male progeny has been developed from Date plum（D. lotus L.）. However，it remained undetermined whether this marker can be employed to identify Diospyros L. and its hybrids. In this study，phenotype observation and DlSx-AF4S genotyping were carried out using 268 accessions，including D. kaki，F1 progenies andsome related species. All of the D. kaki genotypes，with the exception of‘Luotian Tianshi’，‘Xingyi Shuishi’，‘Amahyakume’and‘Shangcheng Dongshi’，displayed consistent results between DlSx-AF4S amplification and sex phenotype. A total of 118 F1 progenies were produced from 11 crosses，among which 51 individuals had flowered. In the flowering plants，DlSx-AF4S profiles of 46（90.2%）plants were identical to the field observation of flower gender. The segregation of DlSx-AF4S in‘Huashi 1’× ‘Luotian Tianshi’F1 population fitted to 1︰1. High transferability of DlSx-AF4S was observed in other species related to D. kaki and D. lotus. Taken together，these results indicated that the DlSx-AF4S marker could be used to distinguish male plants from female ones of persimmon at an early stage.

Key words: Diospyros kaki, DlSx-AF4S, SCAR, male, sex determination