关键词: 苹果, 栽培品种, TP-M13-SSR, 指纹图谱, 分子身份证

Abstract:

A total of 314 apple cultivars from 19 countries all over the world in the national pear and apple repository of Xingcheng were studied with tailed primer M13 microsatellite markers（TP-M13-SSR）. Analysis was made to establish the 314 apple cultivars molecular ID. The results showed that by using 16 selected SSR markers，357 alleles were detected with a mean value of 22.3 alleles. Based on number of alleles and Shannon’s information index，more and more loci were added to distinguish all the apple accessions，and finally six core primers at least were screened to establish germplasms molecular ID. Base on genetic fingerprints at six loci，loci that amplified by each marker were coded，then the code were combined as a molecular ID. Using barcode technology molecular ID can be transferred into barcode ID that can be scaned quickly by machine. Every apple germplasm obtain it’s special molecular ID. The purpose that using least primers distinguishes most apple germplasms has been come true.

Key words: apple, cultivar, TP-M13-SSR, genetic fingerprints, molecular ID