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园艺学报 ›› 2015, Vol. 42 ›› Issue (12): 2526-2534.doi: 10.16420/j.issn.0513-353x.2015-0382

• 研究报告 • 上一篇    下一篇

金叶弯刺蔷薇× 山刺玫遗传连锁图谱的初步构建

杨树华1,李世超1,贾瑞冬1,赵 鑫1,金茂勇2,李凤杰2,葛 红1,*   

  1. 1中国农业科学院蔬菜花卉研究所,北京 100081;2北京市大兴区园林绿化局,北京 102600
  • 出版日期:2015-12-25 发布日期:2015-12-25
  • 基金资助:

    国家‘863’计划项目(2011AA100208);农业部‘948’项目(2011-G17);国家科技支撑计划项目(2012BAD01B0704)北京市大兴区与中国农业科学院科技合作项目;农业部园艺作物生物学与种质创制重点实验室项目

Primary Construction of a Genetic Linkage Map of Rosa beggeriana‘Aurea’× R. Davurica

YANG Shu-hua1,LI Shi-chao1,JIA Rui-dong1,ZHAO Xin1,JIN Mao-yong2,LI Feng-jie2,and GE Hong1,*   

  1. 1Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China;2Forestry and Landscape Bureau of Daxing District of Beijing Municipality,Beijing 102600,China
  • Online:2015-12-25 Published:2015-12-25

摘要:

以金叶弯刺蔷薇(Rosa beggeriana‘Aurea’,叶色突变材料)和山刺玫(R. davurica)为杂交亲本建立F1群体,利用AFLP和SSR分子标记进行遗传连锁图谱构建。结果表明:124个AFLP标记、25个SSR标记及1个形态标记定位于父、母本遗传连锁图谱的13个连锁群上,其中92个分子标记定位于金叶弯刺蔷薇7个连锁群,遗传距离覆盖度为607.2 cM,金叶突变性状被定位于第4连锁群;57个分子标记定位于山刺玫6个连锁群,遗传距离覆盖度为437.1 cM。两类分子标记的总作图效率达到69.6%。发生偏分离的分子标记数量为44个,偏分离率为29.5%。

关键词: 金叶弯刺蔷薇, 山刺玫, 遗传作图, AFLP标记, SSR标记

Abstract:

The F1 population of Rosa beggeriana‘Aurea’× R. davurica was established for the construction of genetic linkage map using AFLP and SSR markers. The results showed that 124 AFLP markers,25 SSR markers and 1 morphological marker were located in total 13 linkage groups of the parents. There were 92 markers covered the genetic distances of 607.2 cM in 7 linkage groups of R. beggeriana‘Aurea’,which the yellow-leaf mutant trait was located in the 4th linkage group. In addition,there were 57 markers covered the genetic distances of 437.1 cM in 6 linkage groups of R. davurica. The total mapping efficiency reached 69.6% and 44 distorted markers were detected with the distortion rate of 29.5%.

Key words: Rosa beggeriana, Rosa davurica, genetic mapping, AFLP, SSR

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