关键词: 大岩桐, 花萼, 幼叶, 转录组, 生物信息学分析

Abstract:

The transcriptome of Sinningia speciosa sepals and young leaves was sequenced by using Illumina Solexa GAⅡx，and the functional annotations and metabolic pathway analyses of some unigenes were conducted in this study. And 22 821 unigenes，longer than 100 bp，were obtained by de novo assembly method. The total scaffold sequence length reached 28.63 Mb with the 1 548 bp of N50，the average unigenes length was 953 bp. Annotation analysis of unigene indicated that 15 053 unigenes had homolog in public protein database；However，7 768 sequences were no hit and might be Sinningia speciosa specific. The study of different KEGG pathways related to hormone metabolism and signaling in sepals and young leaves，inferred that the expression of hormone synthesis genes of promoting growth in sepals is stronger than in leaves，so was the expression of the signal transduction component genes.

Key words: Sinningia speciosa, sepal, young leaf, transcriptome, bioinformation analysis