https://www.ahs.ac.cn/images/0513-353X/images/top-banner1.jpg|#|苹果
https://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner3.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner4.jpg|#|灵芝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner5.jpg|#|桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner6.jpg|#|黄瓜
https://www.ahs.ac.cn/images/0513-353X/images/top-banner7.jpg|#|蝴蝶兰
https://www.ahs.ac.cn/images/0513-353X/images/top-banner8.jpg|#|樱桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner9.jpg|#|观赏荷花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner10.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner11.jpg|#|月季
https://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

园艺学报 ›› 2015, Vol. 42 ›› Issue (12): 2505-2511.doi: 10.16420/j.issn.0513-353x.2015-0649

• 研究报告 • 上一篇    下一篇

四倍体黑芥的获得与鉴定

张巍巍1,刘富强3,轩淑欣2,冯大领1,张成合2,*,申书兴2,*   

  1. 1河北农业大学生命科学学院,河北保定 071000;2河北农业大学园艺学院,河北保定 071000;3保定市农业科学研究院,河北保定 071000
  • 出版日期:2015-12-25 发布日期:2015-12-25
  • 基金资助:

    ‘十二五’农村领域国家科技计划课题(2012AA100202-5);农业科研杰出人才培养计划项目(2130106);国家自然科学基
    金项目(31171964);河北省自然科学基金项目(C2014204135)

Obtaining and Identification of Tetraploid Brassica nigra

ZHANG Wei-wei1,LIU Fu-qiang3,XUAN Shu-xin2,FENG Da-ling1,ZHANG Cheng-he2,*,and SHEN Shu-xing2,*   

  1. 1College of Life Science,Agricultural University of Hebei,Baoding,Hebei 071000,China;2College of Horticulture,Agricultural University of Hebei,Baoding,Hebei 071000,China;3Baoding Academy of Agricultural Sciences,Baoding,Hebei 071000,China
  • Online:2015-12-25 Published:2015-12-25

摘要:

以黑芥为材料进行了同源多倍体的诱导和鉴定研究。结果表明,用0.1%秋水仙素药液处理黑芥籽苗生长点24 ~ 72 h均可诱导获得多倍体植株,其中处理48 h的诱导效果最好,四倍体株率达73.33%。四倍体黑芥长势良好,叶片和花变大,叶柄变短,叶色和花色变深,开花期较二倍体晚2 ~ 3 d。四倍体黑芥能正常开花散粉,但结籽率较低,仅为13.83%。

关键词: 黑芥, 四倍体, 秋水仙素

Abstract:

Inducing tetraploid plants is an effective way to overcome the infertility of distant hybridization. In the present paper,tetraploid inducing and identification of Brassica nigra were conducted. The results showed that treating the seedling apexes of B. nigra with 0.1% colchicine solution for 24–72 h,the tetraploid plants were induced,in which the treatment for 48 hours achieved the highest percentage(73.33%)of tetraploid plants. The tetraploid plants grew and developed normally,their blades and flowers were obviously larger than those of diploid plants. The anthesis of the tetraploid plants was about
2–3 days later than the diploid plants. The seed setting of tetraploid plants was only 13.83%.

Key words: Brassica nigra, autotetraploid, colchicine

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