关键词: 大白菜, 结球甘蓝, 单体异附加系, 抽薹, BrFLCs

Abstract:

Monosomic addition line of Chinese cabbage introduced alien cabbage chromosome 4 had been obtained. In this research，the hybridizations between Chinese cabbage monosomic alien addition line and four Chinese cabbage inbred lines with early or late bolting were developed. These hybrids were identified by chromosome counting of root tip and chromosome 4 specific markers of cabbage compared with Chinese cabbage. Two 2n = 21 and two 2n = 20 plants were obtained in every cross combination. The results of bolting investigation for hybrids indicated that the bolting of plants with chromosome 2n = 21 were later than the plants with 2n = 20 from the same hybridized combination. The gene BrFLCs expression in hybrids and male parents were analyzed by the Real-time PCR. The results showed that the relative expression of BrFLCs was not the only major factor，the effect of genome on bolting and buddingwas more than that only from BrFLCs. The effect of added cabbage chromosome 4 on the BrFLCs expression was influenced by the background of Chinese cabbage genome.

Key words: Chinese cabbage, cabbage, monosomic alien addition line, bolting, BrFLCs