关键词: 茶树, CsSUL3.5, 硒, 克隆, 表达分析

Abstract:

The cDNA of putative sulfate transporter（CsSUL3.5）gene（GenBank accession number
KP984500）was cloned from tea plant[Camellia sinensis（L.）O. Kuntze]. Bioinformatics analysis
indicated that the cDNA of CsSUL3.5 containing 1 914 bp ORF encoded 637 amino acids residues with a
putative molecular mass of 70.38 kD. It was predicted that CsSUL3.5 was a non-secretory protein without
a signal peptide. CsSUL3.5 could be located in the plasma membrane with 11 transmembrane domains.
Further analyses showed that CsSUL3.5 had the typically conserved domain and the highest identity（74%）with Sulfate Transporter 3.5 of Nicotiana sylvestris. The real-time PCR analysis showed that
CsSUL3.5 transcripts were significantly increased upon the treatment of Na2SO4 and Na2SeO4.

Key words: tea plant, CsSUL3.5, selenium, cloning, expression analysis