关键词: 苹果, 炭疽菌叶枯病, 抗性遗传, SSR标记

Abstract:

Genetic study of apple resistance to Glomerella Leaf Spot（GLS）were carried out by using 4 F1 hybrid groups（‘Fuji’בGolden Delicious’，‘Golden Delicious’בFuji’，‘Gala’בFuji’and‘Fuji’בQF-2’）generated from two highly resistant variety or selection，‘Fuji’and‘QF-2’，and two highly susceptible varieties‘Golden Delicious’and‘Gala’. The results showed that the separation ratio of resistant plants to the susceptible ones in 4 F1 hybrid groups were statistically consistent with the theoretical ratios of 1︰1，1︰1，1︰0 and 0︰1. By comprehensive analysis，the conclusions could be drawn as the follows：The apple resistance to GLS may controlled by a single recessive gene. The genotype of the resistant plants was rr，while the genotypes of the susceptible ones were RR and Rr. Using‘GoldenDelicious’בFuji’F1 hybrid groups and the Bulked Segregation Analysis（BSA）method，the marker S0506206-243 bp associated with disease resistance character to GLS were identified through screening 500 SSR primers covering the whole apple genome evenly，and the genetic distance between the marker and the resistant gene to GLS was 9.8 cM.

Key words: apple, Glomerella leaf spot, inheritance of resistance, SSR marker