关键词: 悬铃木, 叶片, 下胚轴, 不定芽分化, 组织培养, 全息现象

Abstract: Effects of different genotypes, PGRs, illumination conditions and position of exp lants on adventitious shoot regeneration from hypocotyl and /or leaf of Platanus acerifolia were investigated. The results indicated that the ratio of 62BA to IBA in the medium influenced shoot regeneration of hypocotyl segments and 6-BA ∶IBA < 20 ∶1 gave a better effect; Shoot regeneration from leaf exp lantswas significantly different among four different genotypes investigated, with the highest regeneration rate obtained for leaf exp lants of genotype PH2, and TDZ was less effective than 6-BA to induce shoot regeneration from PH2 leaves. Maintaining the cultures in darkness for the firstmonth promoted shoot regeneration of hypocotyl explants to some extent, but leaves incubated in darkness regenerated less shoots evidently; Light intensity of 50 - 100 lx was favorable to shoot regeneration. Significant differences of regeneration capability existed among different sections of hypocotyl and position of leaves, and the hypocotyl section adjacent to the cotyledons and the uppermost two leaves of the shoots showed better regeneration capability than the lower sections and leaves. Furthermore, shoot regeneration of the intact leaveswounded by three cutswasmore efficient than that of severed leaf segments, indicating that the ECIWO /holographic phenomenon existed during in vitro culture of hypocotyls and leaves of P. acerifolia.

Key words: Platanus acerifolia, leaf explant, hypocotyl explant, shoot regeneration, tissue culture, holographic phenomenon