大白菜雄性育性转化相关β-酯酶同功酶双向电泳分析

园艺学报 ›› 2008, Vol. 35 ›› Issue (5): 681-686.

大白菜雄性育性转化相关β-酯酶同功酶双向电泳分析

张少丽;张鲁刚*;张明科

（西北农林科技大学园艺学院, 农业部西北园艺植物种质资源与遗传改良重点开放实验室,陕西杨凌712100）

收稿日期:2007-11-12 修回日期:2008-04-08 出版日期:2008-05-25 发布日期:2008-05-25
通讯作者: 张鲁刚

Analysis of β-EST Isoenzymes Related to Sterility Changeover in Temperature-sensitive CMS of Chinese Cabbage (Brassica campestris L. ssp. pekinensis) with Two-dimensional GEL Electrophoresis

ZHANG Shao-li,ZHANG Lu-gang*, and ZHANG Ming-ke

( College of Horticulture，Northwest A & F University, Key Laboratory of Horticulture Plant Germplasm and Genetic Improvement, Ministry of Agriculture, Yangling, Shaanxi 712100, China )

Received:2007-11-12 Revised:2008-04-08 Online:2008-05-25 Published:2008-05-25
Contact: ZHANG Lu-gang

摘要/Abstract

摘要：

以大白菜温敏雄性不育系TsCMS7311(A)及其保持系7311(B)的小花蕾（长度大约1.5㎜）为材料，活性凝胶电泳分析发现：β-酯酶同功酶与育性转化关系密切，正常不育系比保持系缺少迁移率为0.683、0.718和0.742的3条酶带；随着育性的转化，酶谱接近保持系带型；当育性回复到正常的不育状态时，其同功酶酶谱也随之回复到正常的不育系带型。在低温处理后的不育系TsCMS7311(A^T)育性转化期间，回收与雄性育性转化相关的β-酯酶带区，双向凝胶电泳分析，结果表明，保持系B和转化后的不育系A^T的双向电泳图谱相似，第一向IEF电泳中各获得了两条特异谱带，等电点在7.7附近；第二向SDS电泳中各得到了6种β-酯酶多肽，分子量在55～62 kD之间。

关键词: 大白菜, 育性转化, β-酯酶同功酶, 双向电泳

Abstract:

This paper studied the change of β-EST isoenzymes of small buds (1.5mm or so) in the thermo-sensitive CMS of Chinese Cabbage (Brassica campestris L. ssp. pekinensis) TsCMS7311(A) during the fertility changeover. By one-dimensional GEL electrophoresis analyzing, we found that there were three β-EST isoenzymes related to the sterility changeover closely, their Rf were 0.683, 0.718 and 0.742 respectively. During sterile stamen changed into fertile in male sterile line(AT) after low temperature treatment, the (-EST zymograms changed into maintainer line's(B) too；While fertile stamen in male sterile line (AT) came back into sterile, the (-EST zymograms came back into the natural male sterile line's (A) again. Enriched these β-EST isoenzymes with electroelution, then separated and purified theseβ-EST isoenzymes with two-dimensional GEL electrophoresis, we gained six polypeptides, whose pI values were about 7.7 and molecular weight were between 55 kD and 62 kD.

Key words: Chinese cabbage, sterile changeover, (-EST isoenzymes, two-dimensional GEL electrophoresis

中图分类号:

S 634.1

张少丽;张鲁刚;张明科. 大白菜雄性育性转化相关β-酯酶同功酶双向电泳分析[J]. 园艺学报, 2008, 35(5): 681-686.

ZHANG Shao-li;ZHANG Lu-gang;and ZHANG Ming-ke. Analysis of β-EST Isoenzymes Related to Sterility Changeover in Temperature-sensitive CMS of Chinese Cabbage (Brassica campestris L. ssp. pekinensis) with Two-dimensional GEL Electrophoresis[J]. ACTA HORTICULTURAE SINICA, 2008, 35(5): 681-686.

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