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园艺学报 ›› 2015, Vol. 42 ›› Issue (8): 1457-1466.doi: 10.16420/j.issn.0513-353x.2015-0258

• 果树 • 上一篇    下一篇

砂梨山梨醇转运蛋白(SOT)基因家族成员表达特性及在果实糖积累中的作用初探

戴美松,徐飞,施泽彬,徐昌杰   

  1. 1浙江大学农业与生物技术学院,浙江省园艺植物整合生物学研究与应用重点实验室,杭州 310058;2浙江省农业科学院园艺研究所,杭州310021;3浙江省农业科学院数字农业研究所,杭州 310021
  • 出版日期:2015-08-25 发布日期:2015-08-25
  • 基金资助:
    浙江省自然科学基金项目(LY12C15007);国家现代农业产业技术体系建设专项资金项目(CARS-09-04);浙江省果品农业新品种选育重大科技专项(2012C12904-2)

Preliminary Study on Expression Characteristics of Sorbitol Transporter (SOT)Gene Family and the Role in Sugar Accumulation in Pyrus pyrifolia Fruits

DAI Mei-Song, XU Fei, SHI Ze-Bin, XU Chang-Jie   

  1. 1Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology,College of Agriculture and Biotechnology,Zhejiang University,Hangzhou 310058,China;2Institute of Horticulture,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China;3Institute of Digital Agriculture,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China
  • Online:2015-08-25 Published:2015-08-25

摘要: 山梨醇转运蛋白(SOT)是植物山梨醇运输的关键载体。参考已公布的白梨基因组数据,应用RNA-seq技术在砂梨果肉中鉴别出22个有表达的山梨醇转运蛋白(SOT)基因家族成员,其中10个高表达成员的表达量之和占总表达量的92%。qRT-PCR分析表明上述10个成员的表达存在明显的组织特异性,所有成员在种子中的表达丰度最低;PpySOT8在所有组织和器官中都有不同程度表达;PpySOT26仅在幼叶中有一定表达。在果实发育期间PpySOT2、PpySOT8、PpySOT10/28和PpySOT33的相对表达丰度与果实山梨醇积累呈现良好相关性。4 ℃贮藏期间果实山梨醇含量趋于下降,与PpySOT3、PpySOT4、PpySOT8、PpySOT25、PpySOT32和PpySOT33表达上调相关。

关键词: 砂梨, 山梨醇转运蛋白(SOT), 基因家族, 表达特性, 果实,

Abstract: Sorbitol transporter(SOT)is an essential carrier for sorbitol transport in plants. A total of 22 expressed PpySOT members were identified from sand pear(Pyrus pyrifolia Nakai.)flesh by a RNA-seq analysis using Chinese white pear(P. bretschneideri Rehd.)genome as reference,with the top 10 members accounting for 92% of total transcript abundance. Tissue-specific expression patterns of these 10 members were observed via qRT-PCR. All members barely expressed in seed,PpySOT8 expressed in all tissues analysed,while the expression of PpySOT26 was mostly restricted in young leaves. The transcriptabundance of PpySOT2,PpySOT8,PpySOT10/28 and PpySOT33 was in a close positive correlation with sorbitol accumulation during fruit development. The content of sorbitol in flesh decreased during cold storage at 4 ℃,and it was related to the increased expression of PpySOT3,PpySOT4,PpySOT8,PpySOT25,PpySOT32 and PpySOT33.

Key words: Pyrus pyrifolia, sorbitol transporter(SOT), gene family, expression characteristic, fruit, sugar

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