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园艺学报 ›› 2015, Vol. 42 ›› Issue (8): 1448-1456.doi: 10.16420/j.issn.0513-353x.2015-0030

• 果树 • 上一篇    下一篇

库尔勒香梨kfpMYB及其启动子的克隆和对激素的应答反应

王博慧,孙晓霞,牛建新   

  1. 石河子大学农学院,特色果蔬栽培生理与种质资源利用兵团重点实验室,新疆石河子 832003
  • 出版日期:2015-08-25 发布日期:2015-08-25
  • 基金资助:
    国家自然科学基金项目(31360474);高等学校博士学科点专项科研基金博导类联合资助课题(2013651810002)

Cloning of kfpMYB Gene and Its Promoter in Korla Fragrant Pear(Pyrus sinkiangensis)and Determination Their Responses to Hormones

WANG Bo-Hui, SUN Xiao-Xia, NIU Jian-Xin   

  1. College of Agriculture,Shihezi University,Xinjiang Production and Construction Crops Key Laboratory of Special Fruits and Vegetables Cultivation Physiology and Germplasm Resources Utilization,Shihezi,Xinjiang 832003,China
  • Online:2015-08-25 Published:2015-08-25

摘要: 为明确库尔勒香梨萼片脱落和宿存与kfpMYB基因表达的关系,以其花器官为材料,根据库尔勒香梨kfpMYB基因cDNA序列及梨基因组信息设计引物,通过PCR获得了该基因长度为1 659 bp的基因组DNA序列和2 440 bp的上游调控序列,利用PLACE和PlantCare数据库进行启动子顺式作用元件的预测分析。生物信息学分析表明,kfpMYB启动子序列中存在一些与激素调节相关的元件,如脱落酸响应顺式作用元件ABRERATCAL、DPBFCOREDCDC3和EBOXBNNAPA,赤霉素信号抑制因子WRKY71OS、GARE-motif和TATC-box,生长素诱导有关元件NTBBF1ARROLB和TGA-element。利用实时荧光定量PCR检测了不同生长调节物质处理对kfpMYB转录水平的影响,实时荧光定量PCR分析结果表明ABA、ETH、GA、NAA和果树促控剂(PBO)对kfpMYB的表达均有不同程度的影响,说明这些调控元件参与了基因的表达。

关键词: 库尔勒香梨, MYB基因, 启动子, 激素, 调控元件

Abstract: The flowers of Korla Fragrant pear(Pyrus sinkiangensis Yu)were used as materials to make sure the relationship between Korla Fragrant pear fruit calyx shed or persistence and the expression of the kfpMYB gene,according to kfpMYB gene’s cDNA sequence and pear genomic information designed a pair of primers,a genomic DNA sequence with a length of 1 659 bp and an upstream regulatory sequence with a length of 2 440 bp were obtained by PCR,the promoter’s cis-acting elements were analyzed and predicted using PLACE and PlantCare databases. Bioinformatics analysis showed that the kfpMYB promoter sequence included transcriptional regulatory elements related to hormonal regulation,including three ABA-responsive cis-acting elements(ABRERATCAL,DPBFCOREDCDC3,and EBOXBNNAPA),three GA-responsive elements(WRKY71OS,GARE-motif,and TATC-box),and two auxin-responsiveelements(NTBBF1ARROLB and TGA-element). Real-time PCR was used to determine the effects of different hormones and growth regulators on the transcriptional level of the kfpMYB gene. The results showed that ABA,ETH,GA,NAA and fruit control agent(PBO)affected the expression of the kfpMYB gene. In conclusion,the results indicate that transcriptional regulatory elements are involved in the expression of the kfpMYB gene.

Key words: Pyrus sinkiangensis, MYB gene, promoter, hormone, regulatory element

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