关键词: 荔枝, 类甜蛋白基因, 基因克隆, 基因表达

Abstract: The full length of cDNA sequence and the genome sequence corresponding to the open reading frame（ORF）of a thaumatin-like protein gene（LcTLP，Accession No：JF682821）were obtained from‘Feizixiao’litchi（Litchi chinensis Sonn.‘Feizixaio’）by PCR method. The cDNA sequence was contained an ORF with 672 bp in length，encoding a polypeptide with 223 amino acid residues. There was no intron in LcTLP genome sequence. Quantitative real-time PCR results showed that the expression of LcTLP was the highest in the flower，followed by in the pericarp，and the lowest in the pulp. During the fruit development，the expression of LcTLP in the pericarp increased firstly，and then decreased. The expression of the gene in the pericarp of postharvested fruit was higher than that of developed fruit. The expression of LcTLP in the pericarp could be induced by Colletotrichum infection；However，it could be inhibited by water loss and low temperature.

Key words: litchi, thaumatin-like protein, gene cloning, gene expression