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园艺学报 ›› 2015, Vol. 42 ›› Issue (7): 1329-1337.doi: 10.16420/j.issn.0513-353x.2015-0249

• 蔬菜 • 上一篇    下一篇

大白菜—结球甘蓝1号染色体二体异附加系自交后代InDel标记分析和染色体数鉴定

李岩宾,轩淑欣,王彦华,冯大领,赵建军,申书兴   

  1. 1河北农业大学园艺学院/河北省蔬菜种质创新与利用重点实验室;2河北农业大学生命学院,河北保定071000
  • 出版日期:2015-07-25 发布日期:2015-07-25
  • 基金资助:

    国家自然科学基金项目(31171964);‘十二五’农村领域国家科技计划课题项目(2012AA100202-5);农业科研杰出人才培养计划项目(2130106);河北省百人计划项目(E2013100011);河北省教育厅项目(QN2014025)

Analysis of InDel Markers and Identification of Chromosome Number in Selfing Progenies of Chinese Cabbage–Head Cabbage Chromosome 1 Disomic Alien Addition Line

LI Yan-Bin, XUAN Shu-Xin, WANG Yan-Hua, FENG Da-Ling, ZHAO Jian-Jun, SHEN Shu-Xing   

  1. 1College of Horticulture,Agricultural University of Hebei,Key Laboratory for Vegetable Germplasm Enhancement and Utilization of Hebei;2College of Life Science,Agricultural University of Hebei,Baoding,Hebei 071000,China
  • Online:2015-07-25 Published:2015-07-25

摘要:

在进行异附加系鉴定和相应连锁群InDel标记筛选的基础上,利用InDel标记和细胞学方法对大白菜—结球甘蓝1号染色体二体异附加系自交后代46个单株进行了鉴定分析。结果表明:结球甘蓝C03连锁群相对于大白菜特异的67个InDel标记,均至少在1份后代单株中扩增出了甘蓝的特异条带;在46个后代单株中,3个单株为染色体数22条的二体异附加系,6个单株为染色体数21条的单体异附加系,1个单株为染色体数20条的大白菜二体代换系,28个单株为染色体数20条的附加了结球甘蓝染色体片段的大白菜易位系,还有3个单株染色体数为22条,2个单株染色体数为21条,推断其分别为易位了结球甘蓝染色体片段的大白菜非整倍体易位系,还有3个单株没有检测到甘蓝特异条带。本研究结果为这些材料的进一步研究和应用奠定了基础。

关键词: 大白菜, 结球甘蓝, 二体异附加系, InDel标记, 染色体

Abstract:

Based on the identification of Chinese cabbage–head cabbage addition lines and the screening of InDel markers on the corresponding linkage groups,46 selfing offspring individuals of Chinese cabbage - head cabbage chromosome 1 disomic alien addition lines were identified and analyzed using InDel markers and cytological method in order to clarify their chromosomal organization and number. The results showed that the specific fragment of head cabbage C03 chromosome could be amplified by each of 67 InDel markers corresponding to C03 linkage group of head cabbage in one individual plant at least. Of 46 selfing offsprings,three individual plants with 22 chromosomes were Chinese cabbage–head cabbage chromosome 1 disomic alien addition lines,six individual plants with 21 chromosomes were Chinese cabbage–head cabbage monosomic addition lines,one individual plant with20 chromosomes was Chinese cabbage–head cabbage disomic substitution line,twenty-eight individual plants with 20 chromosomes were Chinese cabbage translocation lines adding chromosomal C03 fragments of head cabbage. In addition,there were three individual plants with 22 chromosomes and two individual plants with 21 chromosomes,which were inferred to be Chinese cabbage aneuploids with the translocation fragments of head cabbage C03 chromosome. And three individual plants were not detected the specific bands of cabbage. This study will offer useful information for further research and utility of these selfing offsprings.

Key words: Chinese cabbage, head cabbage, disomic alien addition line, InDel markers, chromosome

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