https://www.ahs.ac.cn/images/0513-353X/images/top-banner1.jpg|#|苹果
https://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner3.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner4.jpg|#|灵芝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner5.jpg|#|桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner6.jpg|#|黄瓜
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https://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

园艺学报 ›› 2015, Vol. 42 ›› Issue (7): 1260-1272.doi: 10.16420/j.issn.0513-353x.2015-0166

• 果树 • 上一篇    下一篇

基于microRNA深度测序的猕猴桃性别分化初探

闫明科,徐强,刘春燕,张琼,姚小洪   

  1. 1中国科学院武汉植物园,中国科学院种质创新与特色农业重点实验室,武汉 430074;2中国科学院大学,北京100049;3上饶职业技术学院,江西上饶 334109
  • 出版日期:2015-07-25 发布日期:2015-07-25
  • 基金资助:
    国家自然科学基金项目(31370251)

Preliminary Investigation on Sex Differentiation of Actinidia chinensis by High-throughput microRNAs Sequencing

YAN Ming-Ke, XU Qiang, LIU Chun-Yan, ZHANG Qiong, YAO Xiao-Hong   

  1. 1Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture,Wuhan Botanical Garden,Chinese Academy of Sciences,Wuhan 430074,China;2University of Chinese Academy of Sciences,Beijing 100049,China;3Shangrao Vocational & Technical College,Shangrao,Jiangxi 334109,China
  • Online:2015-07-25 Published:2015-07-25

摘要: 利用新一代高通量测序技术,对猕猴桃雌花和雄花中表达的小RNA进行了测序,分别得到雌花18 408 610条序列和雄花11 191 469条序列。通过生物信息学分析,共鉴定和预测得到39个保守miRNA家族和400个新的miRNA家族,其中有170个miRNA家族在雌、雄花样品间显著差异表达。对差异表达miRNA进行靶基因的预测及注释,结果显示,靶基因产物具有包含核苷三磷酸水解酶的磷酸环结构域的miRNA数量最多。在猕猴桃25号连锁群(Chr25)上共预测得到3个miRNA,其中novel-ach-miR362的靶基因Achn298021可能与猕猴桃花的性别发育有关。

关键词: 猕猴桃, microRNA, 深度测序, 性别分化

Abstract: The miRNAs in female and male flowers of kiwifruit were sequenced by next-generation sequencing,and received 18 408 610 and 11 191 469 reads,respectively. Using bioinformatics methods,39 conserved and 400 novel miRNA families including 170 miRNA families that were differently expressed between the two samples were identified. Targets prediction and annotation of identified miRNAs revealed the most enrichment of P-loop containing nucleoside triphosphate hydrolase domain in InterPro terms. Three miRNAs were predicted to be located on the kiwifruit sex chromosome(Chr25),among which,target gene Achn298021 of novel-ach-miR362 was predicted to be related to flower development,and should be drawn special attention in future study.

Key words: kiwifruit, microRNA, high-throughput sequencing, sex determination

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