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园艺学报 ›› 2015, Vol. 42 ›› Issue (7): 1233-1240.doi: 10.16420/j.issn.0513-353x.2014-1106

• 果树 • 上一篇    下一篇

‘巨峰’葡萄细胞分裂素氧化酶基因CKX3的克隆与表达分析

余义和,李秀珍,陈迪新,李桂荣,李学强,张国海   

  1. 1河南科技大学林学院,河南洛阳 471003;2河南科技学院园艺园林学院,河南新乡 453003
  • 出版日期:2015-07-25 发布日期:2015-07-25
  • 基金资助:
    河南省教育厅科学技术研究重点项目(14A210018);河南科技大学博士科研基金项目(09001765)

Isolation and Expression Analysis of Cytokinin Oxidase Gene CKX3 in Kyoho Grapevine

YU Yi-He, LI Xiu-Zhen, CHEN Di-Xin, LI Gui-Rong, LI Xue-Qiang, ZHANG Guo-Hai   

  1. 1College of Forestry,Henan University of Science & Technology,Luoyang,Henan 471003,China;2School of Horticulture Landscape Architecture,Henan Instiute of Science and Technilogy,Xinxiang,Henan 453003,China
  • Online:2015-07-25 Published:2015-07-25

摘要: 结合同源克隆和RACE技术在‘巨峰’葡萄(Vitis labruscana Bailey × V. vinifera L.‘Kyoho’)中克隆了细胞分裂素氧化酶基因CKX3,分析了该基因的表达特性及其对细胞分裂素的响应。序列分析表明,该基因cDNA全长为2 049 bp(GenBank登录号:KP689597),ORF(开放阅读框)为1 569 bp,编码522个氨基酸,具有FAD(黄素腺嘌呤二核苷酸)结合结构域和细胞分裂素结合结构域。该基因定位在葡萄的7号染色体上,具有4个内含子,5个外显子。氨基酸序列多重比对和系统进化树分析显示‘巨峰’葡萄CKX3与荷花NuCKX3亲缘关系最近,与毛果杨同源性较高。基因表达分析结果显示‘巨峰’葡萄CKX3主要在根部和花序中大量表达,其次是在卷须和果实中有较高的表达,在茎和叶中的表达量较低;在花序发育过程中,在盛花期前表达量较低,盛花期以及盛花后表达量增加。在6-BA处理葡萄叶片后,CKX3的表达与细胞分裂素氧化酶的活性都高于对照。

关键词: 葡萄, 细胞分裂素氧化酶, CKX3, 表达分析

Abstract: Combined with the homologous clone and RACE technology to clone the cytokinin oxidase gene CKX3 in Kyoho grapevine,and analyzes the characteristics of the gene expression and its response to cytokinin. Sequence analysis indicated that CKX3 consisted of 2 049 bp,with ORF of 1 569 bp,encoded 522 amino acids. The deduced amino acids possessed FAD-binding and cytokinin-bining domains. CKX3 gene is located on chromosome 7 in grapevine,this gene contains 4 introns and 5 exons. Multiple sequence alignments and phylogenetic analysis showed that CKX3 had the highest evolutionary relationship with NuCKX3 from Nelumbo nucifera,and had the highest homology with PtCKX3 from Populus trichocarpa. The results at transcriptional level indicated that expression of CKX3 was main in root and inflorescence,the expression in tendril and fruit tissues was higher than that of stem and leavestissues. In inflorescence development process,the expression of CKX3 in blooming before is low,the expression in blooming and blooming later is increased. In the grapevine leaves after 6-BA treatment,the expression of CKX3 and the cytokinin oxidase enzyme activity were higher than that of control.

Key words: grapevine, cytokinin oxidase, CKX3, expression analysis

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