关键词: 水茄, StUBCc, 基因克隆, 表达分析

Abstract: An EST F290 was from suppression subtractive hybridization library made from pathogen treated roots of the seedling roots of verticillium wilt（Verticillium dahliae Kleb）resistant Solanum torvum Swartz. A full length cDNA（756 bp）was obtained using rapid amplification of cDNA ends（RACE）technology，which encoded the ubiquitin-conjugating enzyme E2-2（UBC2）family gene and was designated as StUBCc. GenBank number is KP330492. The putative protein contains of 152 amino acids with a molecular weight of 63.0925 kD and a theoretical pI of 5.13. Multiple sequence alignment of the amino acid sequence between StUBCc and other homologs from several plants were analyzed by MEGA 5 and shows that the StUBCc has higher identity with UBC genes from Vitis vinifera. The identities is 99% with a deduced amino acid sequence of highly conserved functional domain. The expressions of the isolated StUBCc in the roots of S. torvum seedlings treated by verticillium wilt fungus as well as sterilewater at different time points was studied by using quantitative RT-PCR. The results indicated that the expression of the newly isolated StUBCc gene was induced by verticillium wilt infection and was 6.79 times more than the comparison at 6 hours post infection.

Key words: Solanum torvum, StUBCc, gene cloning, expression analysis