关键词: 葡萄, 叶片衰老, 红光和蓝光, 活性氧, 代谢

Abstract: To investigate the effects of red light and blue light on leaf senescence and active oxygen metabolism，the two grape variety Vitis vinifera‘Italia’and V. vinifera‘Centenial Seedless’with different senescence-types cultured in protected and delayed cultivation were studied.‘Italia’and‘Centenial Seedless’were exposed to red light and blue light，and no supplemental lighting as control，and the physiological indexes of their leaves，such as chlorophyll and soluble protein contents，the net photosynthetic rate，superoxide radical（）and hydrogen peroxide（H2O2）contents，malondialdehyde（MDA）accumulation，superoxide dismutase（SOD），peroxidase（POD）and catalase（CAT）activities were determined during natural senescence. Compared with control，red light delayed the decrease ofchlorophyll and soluble protein contents and the net photosynthetic rate significantly，increased the CAT and SOD activities，decreased the MDA， and H2O2 contents，delayed the appearance time of maximum content of H2O2 and postponed the senescence of functional leaves；While blue light significantly decreased the chlorophyll content，the net photosynthetic rate and activity of antioxidant enzymes at early stages of leaf senescence，accelerating the senescence process，after that the chlorophyll content，the net photosynthetic rate and activity of antioxidant enzymes in the blue light treatment were gradually higher than that in the control，so blue light could delay leaf senescence to a certain extent at late sampling stage. There were differences in different senescence-types of grape leaves，the leaves of‘Italia’aged slower and maintained higher antioxidant enzymes activity at the late growth stage.

Key words: grape, leaf senescence, red light and blue light, reactive oxygen, metabolism