桃黄化病病原植原体的鉴定及16S rDNA序列分析

园艺学报 ›› 2009, Vol. 36 ›› Issue (3): 341-346.

桃黄化病病原植原体的鉴定及16S rDNA序列分析

黄　云^1*;王　靖¹;赵艳琴²;何　苗¹

(¹四川农业大学植物病理系, 四川雅安625014; ²内蒙古民族大学植保系, 内蒙古通辽028043)

收稿日期:2008-08-26 修回日期:2008-12-15 出版日期:2009-03-25 发布日期:2009-03-25
通讯作者: 黄　云

The Identif ica tion and Sequence Analysis of 16S Ribosomal DNA of the Phytoplasma sp. Associated with Peach Yellow Disease

HUANG Yun^1*, WANG Jing¹,ZHAO Yan-qin²,and HE Miao¹

(¹Departm ent of Plant Pathology, S ichuan AgriculturalUniversity, Yapan, Sichuan 625014, China; ²Departm ent of Plant Protection, Inner Mongolia University for the N ationalities, Tongliao, InnerM ongolia 028043, China )

Received:2008-08-26 Revised:2008-12-15 Online:2009-03-25 Published:2009-03-25
Contact: HUANG Yun

摘要/Abstract

摘要： 对四川省成都市龙泉驿区桃植原体黄化病病原进行了研究。经DAP I荧光染色, 在荧光显微镜下观察到病叶叶脉韧皮部存在荧光点; 在透射电镜下病叶韧皮部存在植原体菌体, 其大小为40～650 nm, 平均大小350 nm, 单位膜厚度10～16 nm。利用植原体16S rDNA通用引物对桃植原体黄化病患病植株提取的DNA进行nested2PCR扩增, 获得115 kb的特异片段, 同时构建了16S rDNA系统进化树。桃黄化病植原体序列与油桃黄化NecY2In1 (nectarine yellows) 病原同源性最高。从而探明了桃黄化病病原为植原体及其分类地位。

关键词: 桃, 桃黄化病, 植原体, 病原形态, 系统发育, 16S rDNA

Abstract: The phytop lasma associated with peach yellow disease occurred in Longquanyi District in Sichuan Province was studied. The specific fluorescence dots for the pathogen were observed in phloem cells of leaf veins under a fluorescence microscope by staining with 4, 62diamidino222phenylindole diacetate. Phytoplasma-like particles were also found in the same tissues under a transmission electronic microscope. Its diameter was 40-650 nm, 350 nm on average and the thickness of the unitmembrane of the pathogen was 10 - 16 nm. The 16S rDNA about 115 kb was amplified by nested-PCR with universal p rimers for Phytoplasm a using the total DNA extracted from diseased peach as the temp lates. A phylogenetic tree was constructed based on the sequences of 16S rDNA. The result showed that Phytoplasm a associated with peach yellow disease was closely related to nectarine yellows (NecY2In1).

Key words: peach, peach yellow disease, Phytoplasma, morphology, phylogenetics, 16S rDNA

中图分类号:

S 662.11

黄　云;王　靖;赵艳琴;何　苗. 桃黄化病病原植原体的鉴定及16S rDNA序列分析[J]. 园艺学报, 2009, 36(3): 341-346.

HUANG Yun;WANG Jing;ZHAO Yan-qin;and HE Miao. The Identif ica tion and Sequence Analysis of 16S Ribosomal DNA of the Phytoplasma sp. Associated with Peach Yellow Disease[J]. ACTA HORTICULTURAE SINICA, 2009, 36(3): 341-346.

[1]	翟含含, 翟宇杰, 田义, 张叶, 杨丽, 温陟良, 陈海江. 桃SAUR家族基因分析及PpSAUR5功能鉴定[J]. 园艺学报, 2023, 50(1): 1-14.
[2]	袁馨, 徐云鹤, 张雨培, 单楠, 陈楚英, 万春鹏, 开文斌, 翟夏琬, 陈金印, 甘增宇. 猕猴桃后熟过程中ABA响应结合因子AcAREB1调控AcGH3.1的表达[J]. 园艺学报, 2023, 50(1): 53-64.
[3]	邢柱东, 吕福堂, 郭尚敬, 张演义. 新品种‘聊大红金’桃[J]. 园艺学报, 2023, 50(1): 225-226.
[4]	杨兴旺, 王海波, 王莹莹, 王小龙, 王志强, 刘培培, 刘万春, 王孝娣. 中熟抗寒桃新品种‘中农甘爽’[J]. 园艺学报, 2022, 49(S2): 15-16.
[5]	杨兴旺, 王海波, 王莹莹, 张艺灿, 王宝亮, 刘培培, 史祥宾, 刘万春, 王孝娣. 中熟抗寒桃新品种‘中农白干’[J]. 园艺学报, 2022, 49(S2): 17-18.
[6]	杨兴旺, 刘凤之, 王海波, 王莹莹, 王志强, 史祥宾, 冀晓昊, 刘万春, 王孝娣. 中熟抗寒桃新品种‘中农寒水蜜’[J]. 园艺学报, 2022, 49(S2): 19-20.
[7]	杨兴旺, 刘凤之, 王海波, 王莹莹, 张艺灿, 李鹏, 王小龙, 刘万春, 王孝娣. 晚熟抗寒桃新品种‘中农秋香’[J]. 园艺学报, 2022, 49(S2): 21-22.
[8]	王莹莹, 刘立常, 刘志伍, 杨兴旺, 刘万春, 王孝娣, . 极晚熟桃新品种‘中农冬蜜’[J]. 园艺学报, 2022, 49(S2): 23-24.
[9]	王莹莹, 刘立常, 刘志伍, 杨兴旺, 刘万春, 王孝娣, . 小果油桃新品种‘中农珍珠’[J]. 园艺学报, 2022, 49(S2): 25-26.
[10]	吴延军, 刘庆忠, 陈鸿才, 戚行江, 朱东姿, 郑家祥, 曹学敏, 方丹燕. 甜樱桃新品种‘江南锦’[J]. 园艺学报, 2022, 49(S2): 29-30.
[11]	张晓明, 闫国华, 周宇, 王晶, 段续伟, 吴传宝, 张开春. 甜樱桃砧木新品种‘京春2号’[J]. 园艺学报, 2022, 49(S2): 31-32.
[12]	宋放, 陈奇, 袁炎良, 陈沙, 尹海军, 蒋迎春, . 黄肉猕猴桃新品种‘先沃1号’[J]. 园艺学报, 2022, 49(S2): 47-48.
[13]	齐永杰, 高正辉, 马娜, 王清明, 柯凡君, 陈钱, 徐义流, . 黄肉抗溃疡病猕猴桃新品种‘皖农金果’[J]. 园艺学报, 2022, 49(S2): 49-50.
[14]	张慧琴, 楼国荣, 陆玲鸿, 古咸彬, 宋根华, 谢鸣. 黄肉中华猕猴桃新品种‘金义’[J]. 园艺学报, 2022, 49(S2): 51-52.
[15]	季琳琳, 陈素传, 吴志辉, 常君, 韩文妍, 陶汝鹏. 早花山核桃新品种‘宁国山核桃2号’[J]. 园艺学报, 2022, 49(S2): 53-54.