关键词: 平欧杂交榛, 组织培养, 快速繁殖

Abstract: Stem segment and root tiller explants of hybrid hazelnut cultivar Dawei, Jinling, Yuzhui, Pingdinghuang and Bokehong were used to select proper basic medium, establish buds inducement and rapid propagation procedure. The results showed that: (1) during primary culture process, stem segment and root tiller explants were cultured in DKW medium, and the contaminate rate was above 75% and below 20%, survive rate of explants were below 15% and above 65%, propagation times were below 0.3 and above 1.5 respectively. (2) Among basic medium NRM、DKW、WPM、NN and MS, shoot length and auxiliary buds per shoot in NRM medium were higher apparently compared with other medium. (3) Medium NRM+5 mg·L^-1 6-BA+0.01 mg·L^-1 IBA+64.42 mg·L^-1 putrescine+58.10 mg·L^-1 spermidine + 20.20 mg·L^-1 spermine were profitable to promote buds germination and growth. (4) tube plantlet could root in 15 d and rooting rate reached over 95% when its end dipped in 1 g·L^-1 IBA for 20 seconds, and its roots were strong. Transplant survive rate was over 97%.

Key words: Hybrid hazelnut, tissue culture, rapid propagation