切花非洲菊多倍体诱变初报

园艺学报 ›› 2009, Vol. 36 ›› Issue (4): 605-610.

切花非洲菊多倍体诱变初报

李　涵^1,2,4;鄢　波¹; 张　婷^1,4;蒋亚莲^1,4; 张　颢^1,4;于丽霞³;李绅崇^1,4*

(¹云南省农业科学院花卉研究所, 昆明650205; ²云南大学生命科学院, 昆明650050; ³西南林学院, 昆明650224;⁴云南省花卉技术工程中心, 昆明650205)

收稿日期:2008-08-12 修回日期:2009-03-02 出版日期:2009-04-25 发布日期:2009-04-25
通讯作者: 李绅崇

Preliminary Studies on Polyploidy Mutation of Cut Flower Gerbera jamesonii Bolus

LI Han^1,2,4, YAN Bo¹, ZHANG Ting^1,4,JIANG Ya-lian^1,4,ZHANG Hao^1,4, YU Li-xia³, and LI Shen-chong^1,4*

(¹Flower Research Institute, Yunnan Academy of Agricultural Sciences, Kunm ing 650205, China; ²Life Science College, Yunnan University, Kunming 650050, China; ³Southwest Forestry College, Kunm ing 650224, China; ⁴Yunnan Flower Research and Development Center, Kunming 650205, China)

Received:2008-08-12 Revised:2009-03-02 Online:2009-04-25 Published:2009-04-25
Contact: LI Shen-chong

摘要/Abstract

摘要： 以非洲菊‘阳光海岸’和‘白马王子’ (2n=2x=50) 品种为供试材料, 采用秋水仙素对其
组培丛生芽进行离体诱导, 成功获得了四倍体植株。秋水仙素处理浓度和处理时间分别为0.02%、0.05%、0.10%和24、36、48 h。试验结果表明, 以0.10%的秋水仙素处理48 h诱导效果最佳, ‘阳光海岸’和‘白马王子’的丛生芽变异率分别为16%和10%。总体上看, 非洲菊对秋水仙素的敏感性较低。经气孔鉴定、染色体计数和形态学观察, 变异材料染色体数加倍(2n=4x=100) 。与二倍体相比, 变异材料气孔面积增大, 花序变大, 叶色变深, 叶尖变钝圆, 叶片质感变厚, 花梗直径变粗, 花梗基部花青素着色变深。最终分别获得稳定的‘阳光海岸’和‘白马王子’多倍体植株200余株和300余株。

关键词: 非洲菊, 多倍体, 倍性鉴定, 诱变

Abstract:

Tetrap loidswere successfully induced from in vitro plantlets of diploid Gerbera jamesoniiBolus‘Cabana’and‘Dalma’(2n=2x=50) by dipping shoot tip s with colchicine. Colchicine at a concentration of 0.02% , 0.05% , 0.10% were tested for 24, 36 and 48 h. The results indicated that the most efficient
condition for inducing tetrap loids was treated with 0110% colchicine for 48 h. The highest mutation rate of‘Cabana’and‘Dalma’was 16% and 10%, respectively. As a whole, gerbrea shoots seemed be not sensibility to colchicines. Mutation budswere screened bymorphological app raisal, stomatal and cytological identification. The chromosome number of part mutants have doubled to 100 (2n=4x=100). As compared with diploid, tetraploid of Gerbera jamesonii Bolus was characterized by larger stoma area, larger flower head,thicker leaves color, leaves tend to round, thicker leaves, larger stem diameter and anthocyanin coloration of peduncle baseswere obviously strong. Finally, over 200 and 300 stable tetrap loids of‘Cabana’and‘Dalma’were respectively abtained.

Key words: Gerbera jamesonii Bolus, polyploidy, ploidy level identification, mutation

中图分类号:

S 68211 + 1

李　涵;;鄢　波;张　婷;蒋亚莲;张　颢;于丽霞;李绅崇;. 切花非洲菊多倍体诱变初报[J]. 园艺学报, 2009, 36(4): 605-610.

LI Han;;YAN Bo;ZHANG Ting;JIANG Ya-lian;ZHANG Hao;YU Li-xia;and LI Shen-chong;. Preliminary Studies on Polyploidy Mutation of Cut Flower Gerbera jamesonii Bolus[J]. ACTA HORTICULTURAE SINICA, 2009, 36(4): 605-610.

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