关键词: 森林草莓, 果胶裂解酶基因, 基因克隆, 荧光定量表达分析

Abstract: Primers were designed based on the sequence information of woodland strawberries，and pectate lyase gene（PLA，PLB，PLC）was cloned from‘Ruegen’. Phylogenetic tree is clustered by MEGA 5.0 based on amino acid of pectate lyase gene（Fragaria vesca）and known pectate lyase gene of other plants. At the same time，gene expression levels were analyzed by real-time quantitative PCR method for strawberries on different organs and developmental stages of fruit. The results showed that pectate lyase gene is continuous expression in all organs of strawberry plants. In developing strawberry fruit，pectate lyase gene family was significantly higher than other organs，combined with the results of the phylogenetic tree and the change of pectate lyase activity speculate that pectate lyase gene plays a role in the regulation of strawberry fruit ripening，especially in the late stage of fruit ripening.

Key words: Fragaria vesca, pectate lyase, gene cloning, quantitative expression