关键词: 绣线菊, 低温锻炼, 光合作用, 光合蛋白, 光合能力

Abstract: Spiraea trichocarpa Nakai and S. bumalda‘Gold Mound’，which have different cold resistance ablilty were used as experimental materials，for measuring the change of photosynthetic characteristics，establishing the different expression protein patterns of two species Spiraea in low temperature by the core technology of proteomics and then identifying the species of photosynthetic proteins，investigating the respond mechanism of two species Spiraea to low temperature treatments. The results showed that the photosynthetic capacities of two Spiraea species decreased in low temperature，but their capacities of using weak light increased and the capacities of using strong light decreased，S. trichocarpa Nakai with stronger cold resistance showed a better ability of using weak light than that of S. bumalda‘Gold Mound’with the weaker cold resistance. Seventeen kinds of photosynthetic proteins involved in energy metabolism and transport were identified seperately under different low temperature，the enzymes involved in Calvin cycle，TCA cycle，the pentose phosphate pathway and photophosphorylation of S. bumalda‘Gold Mound’showed obvious up and down regulation，while in S. trichocarpa Nakai only the enzymes involved in Calvin cycle and the proteins of photosynthetic electron transportation in PSⅡ showed obvious up and down regulation in low temperature；The photosynthesis of two species Spiraea were affected by low temperature，the change of the photosynthetic proteins in S. bumalda‘Gold Mound’were more significant.

Key words: Spiraea, low temperature treatment, photosynthesis, photosynthetic proteins, photosynthetic capacities