关键词: 毛葡萄, 水通道蛋白, 干旱胁迫, 克隆, 表达分析

Abstract: A plasma membrane intrinsic proteins（PIPs）gene，designated VhPIP1，was cloned from root on wild grapevine（Vitis heyneana‘Huaxi-9’）native to China by RT-PCR and RACE（Rapid amplication of cDNA ends）. The full cDNA sequence of VhPIP1 was 1 087 bp，containing an open reading frame（858 bp）and encoding a putative VhPIP1 protein with 286 amino acids. Bioinformatics analysis demonstrated that VhPIP1 exhibited an internal symmetry showing two highly conserved NPA（Asn-Pro-Ala）motifs and a typical structure with six membrane-spanning domains and possessing the MIP family signal consensus sequence. Quantitative real-time PCR results showed that the VhPIP1 expressed in roots，stems and leaves，and the expression level was the highest in roots，and the lowest inleaves. Under drought stress，the expression level of VhPIP1 of Huaxi-9 changed with time extension，which was first rised and then dropped，but the expression level of VvPIP1 of Red Globe was decreased. Compared with drought sensitive genotype Red Globe，the relatively higher expression level of VhPIP1 in drought resistance genotype corresponds to the higher leaves relative water content and the lower ion leakage of plasma membrane. These results suggested that the expression quantity of VhPIP1 closely related to drought resistance.

Key words: Vitis heyneana, aquaporin, drought stress, clone, expression