关键词: 杧果, MiHSP17.6, 克隆, 表达分析

Abstract: The full length sequence of cDNA，small heat shock protein 17.6 gene of mango was cloned by RT-PCR and RACE method，which was named MiHSP17.6 with the GenBank accession number of KJ459857. The results showed that the full-length sequence of MiHSP17.6 is 680 bp and the open reading frame is 462 bp，which encoding a polypeptide of 154 amino acids. The untranslated region（UTR）5′ and 3′ with the length of 80 bp and 135 bp，respectively. Comparison of the amino acids sequences of homologous HSP proteins from other species indicated that MiHSP17.6 has a range of 74% to 82% identity. The real-time quantitative PCR results showed that MiHSP17.6 was expressed in all tested organs. And we also found that MiHSP17.6 was regulated by several treatments，such as high temperature（44 ℃），low temperature（4 ℃），salt（NaCl），polyethylene glycol（PEG），abscisic acid（ABA），hydrogenperoxide（H2O2）and salicylic acid（SA）treatments. These results showed that the function of MiHSP17.6 gene has an important role in mango fruit development and stress responses.

Key words: Mangifera indica, MiHSP17.6, cloning, expression analysis