关键词: 换锦花, MYB基因, 克隆, 表达分析

Abstract: A MYB gene named LsMYB4 was cloned by RT-PCR and RACE methods from petals of Lycoris sprengeri. Sequences analysis showed that the full-length MYB cDNA was 793 bp and contained a ORF of 606 bp encoding 201 amino acids. The LsMYB4 protein had a conserved R2R3-MYB domain and the amino acids sequence shared up to 96% homelogies with anthycyanin biosynthesis-related R2R3-MYB transcription factor in Narcissus tazetta. The expression analysis by real time qRT-PCR showed that LsMYB4 was expressed in different tissues and flowering periods. The expression level in petal was almost ten times higher than in leaf. And in florescence period the expression of LsMYB4 was twenty times higher than in early budding period. The LsMYB4 expressed discrepantly in four clones of L. sprengeri，and results of it implicated that the lighter flower color，the higher expression level. This showed that LsMYB4 played a possibly negative role in anthocyanin biosynthesis of Lycoris sprengeri.

Key words: Lycoris sprengeri, MYB, cloning, expression analysis