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园艺学报 ›› 2014, Vol. 41 ›› Issue (10): 2107-2114 .

• 研究报告 • 上一篇    下一篇

基于多种DNA序列的霉梨起源初探

郑小艳1,2,滕元文2,*   

  1. 1丽水学院生态学院,浙江丽水 323000;2浙江大学园艺系,农业部园艺植物生长发育与品质调控重点开放实验室,杭州 310058
  • 收稿日期:2014-06-16 出版日期:2014-10-25 发布日期:2014-10-25
  • 基金资助:

    浙江省自然科学基金项目(LQ13C020001);国家自然科学基金项目(30871690);高等学校博士学科点专项科研基金项目(20110101110091)

Origin of Meili(Pyrus L.)Based on Data from Multiple DNA Sequences

ZHENG Xiao-yan1,2 and TENG Yuan-wen2,*   

  1. 1College of Ecology,Lishui University,Lishui,Zhejiang 323000,China;2Department of Horticulture,the State Agricultural Ministry Key Laboratory of Horticultural Plant Growth,Development and Quality Improvement,Zhejiang University,Hangzhou 310058,China
  • Received:2014-06-16 Online:2014-10-25 Published:2014-10-25

摘要: 为了探索霉梨(Pyrus spp.)品种群的起源,基于叶绿体片段(accD-psaI,trnL-F)及低拷贝核基因LEY2第二内含子(LFY2int2-N)DNA序列信息对相关的梨属植物进行了系统发育分析。结果表明:除了‘小霉梨’,其它样本均为同一种叶绿体单倍型,与大多数砂梨品种的单倍型一样;基于LFY2int2-N的系统发育树显示霉梨和浙江原产砂梨均非单系类群,但两者关系密切;霉梨并非种间杂种,豆梨很可能没有直接参与其起源。

关键词: 霉梨, DNA序列, 起源, 系统发育

Abstract: To elucidate the origin of Meli pear group,phylogenetic relationships among related Pyrus species were studied based on two noncoding regions of cpDNA(accD-psaI,trnL-F)and one low copy nuclear gene intron(LFY2int2-N). As a result,all Meli cultivars but‘Xiaomeili’,as well as most of the P. pyrifolia cultivars,belong to one cpDNA haplotype;The phylogenetic tree based on LFY2int2-N showed that both Meili and P. pyrifolia are not monophyletic,and Meili is most closely related to P. pyrifolia;Meili is not inter-specific hybrids,P. calleryana probably has not been involved in the origin of Meili. Origin of Meili should be further studied by investigating more samples and nuclear markers.

Key words: Meili(Pyrus spp.), DNA sequence, origin, phylogenetics

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