关键词: 大白菜, SSR, 核心引物, 品种鉴定

Abstract: To screen a core set of SSR primers for varieties identification in Chinese cabbage（Brassica rapa L. ssp. pekinensis），54 elite inbred lines were applied and 51 highly informative SSR markers that were equally distributed in the whole genome and had unique amplification loci，good stability and repeatability，were selected from the 2 129 mapped SSR as the candidate markers. According to results of Polymorphism Information Content（PIC），allele number，physical locations of loci and Principal Component Analysis（PCA）etc.，a core set of 28 SSR primers were further confirmed. Meanwhile，the dendrograms of 54 elite inbred lines based on 28 core primer combinations，51 candidateSSR primers and 123 markers（72 SNP and 51 polymorphic SSR primers）were highly consistent. Moreover，The utility of this core set SSRs was demonstrated in 262 inbred lines，which could be placed into three clusters that were largely consistent with previous classification. Finally，SSR fingerprint of 242 Chinese cabbage varieties were constructed using this core primer combinations. This core set of SSRmarkers should be very useful for genetic variation analysis and DNA fingerprinting of varieties，and would provide technical support for detecting of distinctness，uniformity and stability of new variety in Chinese cabbage.

Key words: Chinese cabbage, SSR, core primer, variety identification