关键词: 柿, DkADH1, 克隆, 基因表达

Abstract: An alcohol dehydrogenase 1（ADH1）gene named DkADH1 was cloned from‘Nantong Xiaofangshi’persimmon fruit using homology gene clone method. The results showed that the full-length of DkADH1 gene was 1 377 bp and the open reading frame（ORF）of DkADH1 was 1 137 nucleotides which encoded 379 amino acids. It has structure domain and function domain of the typical ADH gene. The phylogenetic tree analysis of the evolutionary relationship of ADH between different species indicated that DkADH1 was gathered dicotyledonous plants，which closed to Solanum lycopersicum and Malus × domestica. qRT-PCR was used to analyze the expression patterns of DkADH1 gene and tannin biosynthesis related genes in fruits and various tissues at different developmental stages. The tannin content at different period was also measured which suggested that soluble tannin was gradually decline，but insoluble tannin was gradually rising in the fruit development. DkADH1 expressed in different tissue including stem，leaf，fruit and other organs. It had the highest expression level in peel. The expression was upregulated during the ripening stage of the fruit. Tannin biosynthesis pathway related genes DkF3′5′H and DkMYB4 expression were downregulated in fruits during the ethanol treated，we speculated that DkADH1 could inhibit the expression of tannin biosynthesis pathway genes to reduce the fruit soluble tannin content.

Key words: persimmon, DkADH1, clone, gene expression