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园艺学报 ›› 2014, Vol. 41 ›› Issue (8): 1609-1619.

• 蔬菜 • 上一篇    下一篇

大白菜突变体库的构建及M2叶片表型变异的研究

卢 银,刘梦洋,赵建军,王彦华,罗双霞,轩淑欣,代双燕,王超硕,申书兴*   

  1. (河北农业大学园艺学院,河北省蔬菜种质创新与利用重点实验室,河北保定 071001)
  • 收稿日期:2014-04-29 出版日期:2014-08-25 发布日期:2014-08-25

Construction of One Mutant Library and Research on Phenotypic Variation of M2 Population Leaves in Chinese Cabbage

LU Yin,LIU Meng-yang,ZHAO Jian-jun,WANG Yan-hua,LUO Shuang-xia,XUAN Shu-xin,DAI Shuang-yan,WANG Chao-shuo,and SHEN Shu-xing*   

  1. (Key Laboratory for Vegetable Germplasm Enhancement and Utilization of Hebei,College of Horticulture,Agricultural University of Hebei,Baoding,Heibei 071001,China)
  • Received:2014-04-29 Online:2014-08-25 Published:2014-08-25

摘要: 利用不同浓度甲基磺酸乙酯(EMS)诱变大白菜自交系‘A03’种子,根据M1代植株发芽率、成活率、育性及M2代成活率,筛选出适宜的EMS诱变浓度为0.4%。采取EMS种子诱变1次、EMS种子诱变2次及EMS花蕾诱变结合小孢子培养的方法构建了含有4 253个家系及相应自交M2代种子的大白菜突变体库。在该突变体库M2群体中分别对苗期6 404株幼苗及莲座期7 756个单株的叶片性状进行了形态学调查,总的表型变异频率高达37.62%和26.33%。

关键词: 大白菜, EMS, 突变体库, 诱变

Abstract: Seeds of the Chinese cabbage inbred line‘A03’were treated by EMS with different concentrations in this study. The suitable EMS concentration for seed mutagenesis was determined as 0.4% by studying the germination rate,survival rate,fertility of M1 plants and survival rate of M2 individuals. By three ways of one time seeds treatment with EMS,two times of seeds treatment with EMS,and buds treatment with EMS combined with microspore culture,the Chinese cabbage mutant library contained 4 253 M1 families and their selfing seeds of M2 was constructed. For M2 individuals in the mutant library,leaf phenotypic traits of 6 404 seedlings at seedling stage and 7 756 plants at rosette stage were investigated,resulting in total mutant frequency of 37.62% and 26.33%,respectively.

Key words: Brassica rapa, EMS, mutant library, mutagenesis

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